Sesquiterpene lactone 6‑O‑angeloylplenolin reverses vincristine resistance by inhibiting YB‑1 nuclear translocation in colon carcinoma cells

Li,Changlong; Wu,Hezhen; Yang,Yanfang; Liu,Jianwen; Chen,Zhenwen

doi:10.3892/ol.2018.8592

Sesquiterpene lactone 6‑O‑angeloylplenolin reverses vincristine resistance by inhibiting YB‑1 nuclear translocation in colon carcinoma cells

Authors:
- Changlong Li
- Hezhen Wu
- Yanfang Yang
- Jianwen Liu
- Zhenwen Chen
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Affiliations: School of Basic Medical Science, Capital Medical University, Beijing 100069, P.R. China, Key Laboratory of Resources and Chemistry of Chinese Medicine of the Ministry of Education, Hubei University of Chinese Medicine, Wuhan, Hubei 430061, P.R. China, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, P.R. China
Published online on: April 27, 2018 https://doi.org/10.3892/ol.2018.8592
Pages: 9673-9680
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy efficacy. In the present study, 6‑O‑angeloylplenolin repressed the overexpression of ATP binding cassette subfamily B member 1 (MDR1) and increasing the intracellular concentration of anticancer drugs. A reduction in P‑glycoprotein expression (encoded by MDR1) was observed in parallel with a decline in mRNA expression in vincristine‑resistant HCT (HCT‑8/VCR) cells treated with 6‑O‑angeloylplenolin. In addition, 6‑O‑angeloylplenolin suppressed the activity of the MDR1 gene promoter. Treatment with 6‑O‑angeloylplenolin also decreased the amount of the specific protein complex that interacted with the MDR1 gene promoter in HCT‑8/VCR cells, potentially leading to the suppression of MDR1 expression. Treatment with 6‑O‑angeloylplenolin inhibited the nuclear translocation of Y‑box binding protein‑1 in HCT‑8/VCR cells treated with 6‑O‑angeloylplenolin, contributing to the negative regulation of MDR1. Finally, 6‑O‑angeloylplenolin reversed VCR resistance in an HCT/VCR xenograft model. In conclusion, 6‑O‑angeloylplenolin exhibited a MDR‑reversing effect by downregulating MDR1 expression and could represent a novel adjuvant agent for chemotherapy.

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