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Article

Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells

  • Authors:
    • Ruirui Xu
    • Mingyan Xu
    • Yucai Fu
    • Xiaoling Deng
    • Hui Han
    • Xihe Chen
    • Wenjing He
    • Gengzhen Chen
  • View Affiliations / Copyright

    Affiliations: Minimally Invasive Medical Center, The Second Affiliated Hospital of Shantou Medical College, Shantou, Guangdong 515041, P.R. China, Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China
  • Pages: 439-446
    |
    Published online on: May 8, 2018
       https://doi.org/10.3892/ol.2018.8672
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Abstract

Although it is difficult to detect αvβ6 integrin (αvβ6) in normal epithelia cells, its expression is upregulated during wound healing and carcinogenesis. Overexpression of αvβ6 has been demonstrated in epithelial cell carcinomas, such as adenocarcinoma of the colon and ovary. However, the expression of αvβ6 has not been reported in hepatocellular carcinoma (HCC). We previously indicated that LPA may induce αvβ6‑mediated TGF‑β1 signaling mechanisms during the pathogenesis of lung injury and fibrosis. In addition, transforming growth factor‑β1 (TGF‑β1) and lysophosphatidic acid (LPA) have been demonstrated to participate in the progression of HCC. In the present study, we hypothesized that TGF‑β1 and LPA would serve a key role in the subunit integrin β6 (Itgβ6) transcriptional regulatory mechanism in HCC. It was identified that human HCC tissues and Hep‑3B cells expressed Itgβ6. Treatment of Hep‑3B with TGF‑β1 or LPA increased the expression of Itgβ6. Furthermore, truncation experiments indicated a positive regulatory region at ‑326 to ‑157 bp of the Itgβ6 promoter. TGF‑β1 and LPA increased transcriptional activation at this regulatory region. To the best of our knowledge, the present study was the first to demonstrate Itgβ6 expression in HCC, and the data indicate that TGF‑β1 and LPA regulate Itgβ6 expression through the Itgβ6 gene promoter, which is an important factor in the development of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Xu R, Xu M, Fu Y, Deng X, Han H, Chen X, He W and Chen G: Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells . Oncol Lett 16: 439-446, 2018.
APA
Xu, R., Xu, M., Fu, Y., Deng, X., Han, H., Chen, X. ... Chen, G. (2018). Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells . Oncology Letters, 16, 439-446. https://doi.org/10.3892/ol.2018.8672
MLA
Xu, R., Xu, M., Fu, Y., Deng, X., Han, H., Chen, X., He, W., Chen, G."Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells ". Oncology Letters 16.1 (2018): 439-446.
Chicago
Xu, R., Xu, M., Fu, Y., Deng, X., Han, H., Chen, X., He, W., Chen, G."Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells ". Oncology Letters 16, no. 1 (2018): 439-446. https://doi.org/10.3892/ol.2018.8672
Copy and paste a formatted citation
x
Spandidos Publications style
Xu R, Xu M, Fu Y, Deng X, Han H, Chen X, He W and Chen G: Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells . Oncol Lett 16: 439-446, 2018.
APA
Xu, R., Xu, M., Fu, Y., Deng, X., Han, H., Chen, X. ... Chen, G. (2018). Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells . Oncology Letters, 16, 439-446. https://doi.org/10.3892/ol.2018.8672
MLA
Xu, R., Xu, M., Fu, Y., Deng, X., Han, H., Chen, X., He, W., Chen, G."Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells ". Oncology Letters 16.1 (2018): 439-446.
Chicago
Xu, R., Xu, M., Fu, Y., Deng, X., Han, H., Chen, X., He, W., Chen, G."Transforming growth factor‑β1 and lysophosphatidic acid activate integrin β6 gene promoter in Hep‑3B cells ". Oncology Letters 16, no. 1 (2018): 439-446. https://doi.org/10.3892/ol.2018.8672
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