Prognostic value of hsa‑mir‑299 and hsa‑mir‑7706 in hepatocellular carcinoma

Wang,Fenglin; Dai,Min; Chen,Hongjie; Li,Yue; Zhang,Jiongshan; Zou,Zengcheng; Yang,Hongzhi

doi:10.3892/ol.2018.8710

Prognostic value of hsa‑mir‑299 and hsa‑mir‑7706 in hepatocellular carcinoma

Authors:
- Fenglin Wang
- Min Dai
- Hongjie Chen
- Yue Li
- Jiongshan Zhang
- Zengcheng Zou
- Hongzhi Yang
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Affiliations: Department of Traditional Chinese Medicine, The Third Affiliated Hospital of Sun Yet‑sen University, Guangzhou, Guangdong 510000, P.R. China
Published online on: May 16, 2018 https://doi.org/10.3892/ol.2018.8710
Pages: 815-820

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Abstract

This study aimed to investigate the expression of microRNA (miRNA) 299 and miRNA‑7706 in patients with hepatocellular carcinoma (HCC), and to explore their effects on proliferation of SK‑HEP‑1 HCC cells. Expression of miRNA‑299 and miRNA‑7706 in tumor tissue (HCC group) and adjacent healthy tissue (>30 mm away from the tumor tissue) of 179 patients with HCC was determined by real‑time polymerase chain reaction (qRT‑PCR). miR‑299 mimics and miR‑7706 mimics were transfected into SK‑HEP‑1 HCC cells by RNA transfection. The proliferation and invasion of SK‑HEP‑1 cells were detected by CCK‑8 kit and Transwell kit, respectively. Compared with adjacent tissues, expression levels of miRNA‑299 and miRNA‑7706 in HCC group were significantly downregulated. Analyses on the correlation between the expression of miRNA‑299 and miRNA‑7706 and clinical factors showed that expression levels of miRNA‑299 and miRNA‑7706 were significantly correlated with pathological stages and lymph node metastasis. ROC curve analysis showed that the areas under the curve were 0.837 and 0.845 for miRNA‑299 and miRNA‑7706 in the prediction of HCC, respectively. Survival analysis showed that the 5‑year overall survival rate of patients with high expression levels of miRNA‑299 and miRNA‑7706 was significantly different from that of patients with low expression levels (P=0.016). Compared with cells transfected with scramble mimics, proliferation and invasion abilities of SK‑HEP‑1 cells transfected with miR‑299 mimics and miRNA‑7706 were significantly weakened. Results suggested that downregulation of miRNA‑299 and miRNA‑7706 can inhibit the proliferation of HCC cells and can be used as a new target for the treatment of HCC.

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