MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1

Liu,Wei; Wan,Xiuwei; Mu,Zongyun; Li,Fei; Wang,Lei; Zhao,Jing; Huang,Xiaori

doi:10.3892/ol.2018.8794

MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1

Authors:
- Wei Liu
- Xiuwei Wan
- Zongyun Mu
- Fei Li
- Lei Wang
- Jing Zhao
- Xiaori Huang
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Affiliations: Department of Respiratory Medicine, People's Hospital of Rizhao, Rizhao, Shandong 276826, P.R. China
Published online on: May 24, 2018 https://doi.org/10.3892/ol.2018.8794
Pages: 1708-1714

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Abstract

Mounting evidence has shown that miRNA expression is abnormal in various human cancers. Here, we mainly explored the biological function and the potential mechanisms of miR-1256 in non-small cell lung cancer (NSCLC). The miR-1256 mRNA expression was detected by quantitative real-time PCR and tectonic family member 1 (TCTN1) mRNA expression was detected by immunoblotting. The TCTN1 was identified to be the direct and specific target gene of miR-1256 by luciferase reporter assay. Cell proliferation was examined by methyl thiazolyl tetrazolium assay and migration was detected by transwell assay. MiR-1256 expression was downregulated in NSCLC tissues, whereas the expression of TCTN1 was upregulated, compared with normal tissues. We also found that overexpression of miR-1256 in these NSCLC cell lines inhibited cell proliferation and migration. Furthermore, TCTN1 was identified as a direct target of miR-1256 by luciferase reporter assays. Collectively, these data stated that the inhibitory effect of miR-1256 in NSCLC was realized by upregulating TCTN1, suggesting that miR-1256/TCTN1 axis may play a critical role as NSCLC therapeutic target.

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