Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma

Sun,Guoping; Qiu,Lumei; Cheng,Zhiqiang; Pan,Weibing; Qiu,Jingjun; Zou,Chang; Xie,Ni; Liu,Song; Zhu,Peng; Zeng,Jun; Dai,Yong

doi:10.3892/ol.2018.8800

Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma

Authors:
- Guoping Sun
- Lumei Qiu
- Zhiqiang Cheng
- Weibing Pan
- Jingjun Qiu
- Chang Zou
- Ni Xie
- Song Liu
- Peng Zhu
- Jun Zeng
- Yong Dai
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Affiliations: Core Laboratory, Pingshan New District People's Hospital, Shenzhen, Guangdong 518118, P.R. China, Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China, Core Laboratory, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China
Published online on: May 24, 2018 https://doi.org/10.3892/ol.2018.8800
Pages: 1584-1592
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Complementarity‑determining region 3 (CDR3) of B‑cell receptors (BCRs) and T‑cell receptors are the major site of antigen recognition, which determines a unique clone type, and are considered to be the representative of the disease. In the present study, high‑throughput sequencing was used to analyze the association of characteristics of the BCR immunoglobulin heavy chain (IGH) and the T‑cell receptor β chain (TRB) CDR3 genes in PTC and corresponding pericarcinous tissues from patients. A difference of CDR3 length distributions of total IGH CDR3 sequences between the two groups was revealed. IGHV3‑11/IGHJ6, TRBV2/TRBJ1‑2 and TRBV2/TRBJ1‑1 may be biomarkers for the development of PTC. Furthermore, it was revealed that the extent of the common clonotype expressions at the amino acid level was slightly higher compared with the nucleotide level. The Shannon entropy demonstrated a diversity reduction in PTC compared with the pericarcinous group, and the highly expended clone (HEC) expression of PTC was higher compared with that of the corresponding pericarcinous group. Additionally, the highest clone frequency percentage of IGH and TRB was at 0.1‑1.0% degree of expansion, as HEC expression was higher in PTC compared with the matched group. There was no shared clone of HECs in the two groups either at the amino acid level or at the nucleotide expression level. The differential expression of CDR3 sequences of PTC have been identified in the present study. Further research is required for assessing the immune repertoire size, diversity, cloning tracking and finding public clones of T‑cell and B‑cell populations in the development of PTC.

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