Lily extracts inhibit the proliferation of gastric carcinoma SGC‑7901 cells by affecting cell cycle progression and apoptosis via the upregulation of caspase‑3 and Fas proteins, and the downregulation of FasL protein

Wang,Aihong; Wang,Mingquan; Pang,Qiuxia; Jia,Lei; Zhao,Jumei; Chen,Meini; Zhao,Yufeng

doi:10.3892/ol.2018.8806

Lily extracts inhibit the proliferation of gastric carcinoma SGC‑7901 cells by affecting cell cycle progression and apoptosis via the upregulation of caspase‑3 and Fas proteins, and the downregulation of FasL protein

Authors:
- Aihong Wang
- Mingquan Wang
- Qiuxia Pang
- Lei Jia
- Jumei Zhao
- Meini Chen
- Yufeng Zhao
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Affiliations: School of Medicine, Yan'an University, Yan'an, Shaanxi 716000, P.R. China, Department of Interventional Radiology, Affiliated Hospital of Yan'an University, Yan'an, Shaanxi 716000, P.R. China, Department of Laboratory Medicine, Fuxian County Hospital, Yan'an, Shaanxi 727500, P.R. China
Published online on: May 24, 2018 https://doi.org/10.3892/ol.2018.8806
Pages: 1397-1404
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the effect of alkaloids and carbinol extracts from lily on the proliferation of SGC‑7901 cells, as well as the underlying mechanism. SGC‑7901 cells were incubated with different concentrations of alkaloid or carbinol extracts for 24, 48 or 72 h. MTT assays were used to measure the inhibition rate of SGC‑7901 cell proliferation. Inverted phase contrast and fluorescence microscopy was used to observe morphological changes of SGC‑7901 cells. Flow cytometry was employed to detect cell cycle progression and apoptosis rates of SGC‑7901 cells. Western blotting was performed to measure the expression of caspase‑3, Fas and Fas ligand (FasL) proteins in SGC‑7901 cells. The inhibition rate of SGC‑7901 cell proliferation was significantly enhanced with increasing drug concentrations and time elapsed. Treatment with alkaloid or carbinol extracts deteriorated the morphology of SGC‑7901 cells in a dose‑dependent manner. Alkaloid and carbinol extracts arrested SGC‑7901 cells in the G2/M phase, and induced apoptosis in a dose‑dependent manner. Alkaloid and carbinol extracts enhanced caspase‑3, and Fas expression, but reduced FasL expression in SGC‑7901 cells. The present study demonstrated that alkaloids and carbinol extracts from lily inhibited the proliferation of gastric carcinoma SGC‑7901 cells by arresting cells in the G2/M phase. The upregulation of caspase‑3 and Fas proteins, and the downregulation of FasL protein may be an important mechanism for the induction of SGC‑7901 cell apoptosis.

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