Oncogenic miR‑425‑5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Quan,Jing; Li,Yawen; Pan,Xiang; Lai,Yulin; He,Tao; Lin,Canbin; Zhou,Liang; Zhao,Liwen; Sun,Shuolei; Ding,Yu; Tao,Lingzhi; Hu,Yimin; Wu,Xionghui; Chen,Zebo; Zhang,Fangting; Ye,Jing; Ni,Liangchao; Lai,Yongqing

doi:10.3892/ol.2018.8948

Oncogenic miR‑425‑5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Authors:
- Jing Quan
- Yawen Li
- Xiang Pan
- Yulin Lai
- Tao He
- Canbin Lin
- Liang Zhou
- Liwen Zhao
- Shuolei Sun
- Yu Ding
- Lingzhi Tao
- Yimin Hu
- Xionghui Wu
- Zebo Chen
- Fangting Zhang
- Jing Ye
- Liangchao Ni
- Yongqing Lai
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Affiliations: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Institute of Urology of Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China
Published online on: June 11, 2018 https://doi.org/10.3892/ol.2018.8948
Pages: 2175-2184
Copyright: © Quan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

An increasing number of studies have demonstrated the function of microRNAs (miRNAs) in the initiation and development of various types of cancer. Among them, miR‑425‑5p is proven to serve an important function in several types of cancer, including gastric, cervical cancer, and hepatocellular carcinoma. However, the function of miR‑425‑5p in renal cell carcinoma (RCC) remains unclear. In the present study, it was demonstrated that the expression level of miR‑425‑5p was upregulated in RCC tissues and cell lines compared with normal tissues and cell lines (P<0.05). Additionally, Cell Counting kit‑8 and MTT assays were employed to assess cell viability and proliferation, whereas wound healing and Transwell assays were employed to examine migration and invasion. The results demonstrated that upregulation of miR‑425‑5p promoted cell viability and the invasion and migration of ACHN and 786O cells (P<0.05). Flow cytometric analysis confirmed that upregulation of miR‑425‑5p inhibited apoptosis of ACHN and 786O cells (P<0.05). Downregulation of miR‑425‑5p inhibited the viability and invasion and migration of ACHN and 786O cells (P<0.05). In the present study, upregulation of miR‑425‑5p inhibited apoptosis of ACHN and 786O cells whereas no differences in early apoptotic rate were observed between the inhibitor and inhibitor NC groups for 786O and ACHN cells. These results indicate that miR‑425‑5p may act as an oncogene in RCC.

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