Open Access

Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells

  • Authors:
    • Cheng Wei
    • Chuanrong Chen
    • Yuxin Cheng
    • Lin Zhu
    • Yu Wang
    • Can Luo
    • Yang He
    • Zhiming Yang
    • Zhaoning Ji
  • View Affiliations

  • Published online on: July 6, 2018     https://doi.org/10.3892/ol.2018.9101
  • Pages: 3569-3576
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ailanthone, which is extracted from the traditional Chinese medicinal plant Ailanthus altissima, has been thoroughly demonstrated to have anti‑tumor, anti‑HIV, anti‑inflammatory, anti‑malarial, anti‑allergic and anti‑microbial activities. However, the anti‑proliferative effects of ailanthone on HL‑60 cells and potential mechanisms underlying those effects have not been reported. In the present study, we demonstrated the potent cytotoxicity of ailanthone against HL‑60 cells. Annexin V‑APC/7‑ADD staining assay indicated that ailanthone increased the number of apoptotic cells in a dose‑dependent manner. PI staining showed that ailanthone increased the percentage of G0/G1‑phase cells in a dose‑dependent manner. Acridine orange staining suggested that ailanthone induced the formation of acidic vesicular organelles in HL‑60 cells and pretreatment with BaF‑A1 could attenuate this process. Western blotting showed that ailanthone up‑regulated the protein expression levels of beclin‑1 and LC3‑II and down‑regulated those of LC3‑I and p62 in a dose‑dependent manner. Use of BaF‑A1 showed that the anti‑proliferative effects of ailanthone on HL‑60 cells may be partly attributable to the induction of autophagy‑mediated apoptosis by MTT assay and annexin V‑APC/7‑ADD staining assay.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wei C, Chen C, Cheng Y, Zhu L, Wang Y, Luo C, He Y, Yang Z and Ji Z: Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells. Oncol Lett 16: 3569-3576, 2018.
APA
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C. ... Ji, Z. (2018). Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells. Oncology Letters, 16, 3569-3576. https://doi.org/10.3892/ol.2018.9101
MLA
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C., He, Y., Yang, Z., Ji, Z."Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells". Oncology Letters 16.3 (2018): 3569-3576.
Chicago
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C., He, Y., Yang, Z., Ji, Z."Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells". Oncology Letters 16, no. 3 (2018): 3569-3576. https://doi.org/10.3892/ol.2018.9101