Proteins involved in cutaneous basal cell carcinoma development

Ciążyńska,Magdalena; Bednarski,Igor A.; Wódz,Karolina; Kolano,Paweł; Narbutt,Joanna; Sobjanek,Michał; Woźniacka,Anna; Lesiak,Aleksandra

doi:10.3892/ol.2018.9126

Proteins involved in cutaneous basal cell carcinoma development

Authors:
- Magdalena Ciążyńska
- Igor A. Bednarski
- Karolina Wódz
- Paweł Kolano
- Joanna Narbutt
- Michał Sobjanek
- Anna Woźniacka
- Aleksandra Lesiak
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Affiliations: Department of Proliferative Diseases, Regional Oncology Centre, Łódź 93‑513, Poland, Department of Dermatology, Paediatric Dermatology and Dermatological Oncology, Medical University of Łódź, Łódź 91‑347, Poland, Department of Experimental Immunology, Medical University of Łódź, Łódź 90‑237, Poland, Department of General and Oncological Surgery, Tomaszow Health Centre, Tomaszow Mazowiecki 97‑200, Poland, Department of Dermatology, Venereology and Allergy, Medical University of Gdansk, Gdansk 80‑210, Poland, Department of Dermatology and Venereology, Medical University of Łódź, Łódź 90‑647, Poland
Published online on: July 11, 2018 https://doi.org/10.3892/ol.2018.9126
Pages: 4064-4072

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Abstract

Basal cell carcinoma (BCC) is the most common skin malignancy type in the Caucasian population, with a continuously increasing incidence rate. The etiology of BCC remains unknown, but it appears to have a multifactorial origin resulting from intrinsic and extrinsic factors, including short‑wavelength ultraviolet B radiation. The role of specific proteins in BCC that are known to be responsible for the regulation of cell division and are involved in skin aging, including transforming growth factor (TGF)‑β, Smad2, matrix metalloproteinases (MMPs)‑1, ‑3, ‑8 and ‑9, cathepsin‑K and progerin, remains unknown. The aim of the present study was to assess the mRNA and protein expression profile of samples with diagnosed nodular BCC (nBCC) compared with that of healthy skin samples collected from matched areas. The study group included 22 patients (10 men and 12 women; mean age, 59 years; range, 44‑82 years) with pathologically confirmed nBCC, and 22 healthy volunteers (10 men and 12 women; mean age, 59 years; range, 43‑78 years) as a control group. The expression of the studied proteins was assessed in all samples by western blotting and reverse transcription‑quantitative polymerase chain reaction analysis. Statistically significant increases in the expression of TGF‑β, Smad2, cathepsin‑K, progerin and MMP‑1, ‑3, ‑8 and ‑9 were detected in skin biopsies with diagnosed nBCC compared with the control group, confirming the important role of these proteins in skin carcinogenesis.

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