Mn12Ac inhibits the migration, invasion and epithelial‑mesenchymal transition of lung cancer cells by downregulating the Wnt/β‑catenin and PI3K/AKT signaling pathways

Chen,Zihao; He,Jiangbo; Xing,Xiqian; Li,Ping; Zhang,Wei; Tong,Zhuxiu; Jing,Xiaojie; Li,Licheng; Liu,Dian; Wu,Qiong; Ju,Hongping

doi:10.3892/ol.2018.9136

Mn12Ac inhibits the migration, invasion and epithelial‑mesenchymal transition of lung cancer cells by downregulating the Wnt/β‑catenin and PI3K/AKT signaling pathways

Authors:
- Zihao Chen
- Jiangbo He
- Xiqian Xing
- Ping Li
- Wei Zhang
- Zhuxiu Tong
- Xiaojie Jing
- Licheng Li
- Dian Liu
- Qiong Wu
- Hongping Ju
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Affiliations: Gruaduate School of Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, School of Medicine, Kunming University, Kunming, Yunnan 650214, P.R. China, First Department of Respiratory Medicine, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, Yunnan 650051, P.R. China, Department of Medicine, The People's Hospital of Economic and Technological Development Zone, Kunming, Yunnan 650000, P.R. China, Department of Chemical Science and Technology, Kunming University, Kunming, Yunnan 650214, P.R. China
Published online on: July 11, 2018 https://doi.org/10.3892/ol.2018.9136
Pages: 3943-3948
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lung cancer is the leading cause of global cancer‑associated mortality, therefore it is important to reveal the molecular mechanisms of lung cancer progression and to develop novel therapeutic targets. The results of the present study identified that manganese‑12 acetate (Mn12Ac) was able to significantly inhibit the migration and invasion of A549 cells. Western blotting demonstrated that treatment with Mn12Ac was able to upregulate E‑cadherin, and downregulate N‑cadherin and vimentin. It was also identified by a quantitative polymerase chain reaction analysis that Mn12Ac was able to reduce the mRNA expression levels of EMT‑associated transcription factors Snail, Slug, Twist‑related protein 1 and zinc finger E‑box‑binding homeobox 1. It was also demonstrated that Mn12Ac was able to reduce the expression levels of Wnt and β‑catenin proteins, and suppress the phosphorylation of phosphoinositide 3‑kinase (PI3K) and AKT in A549 cells. Notably, it was revealed that Mn12Ac was able to decrease the mRNA and protein expression levels of programmed death ligand‑1. Taken together, the results suggested that Mn12Ac is able to inhibit cell migration, invasion and EMT in lung cancer cells by regulating the Wnt/β‑catenin and PI3K/AKT signaling pathways.

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