miR‑339‑5p inhibits lung adenocarcinoma invasion and migration by directly targeting BCL6

Li,Peng; Liu,Huaqing; Li,Yanmeng; Wang,Yan; Zhao,Lin; Wang,Hui

doi:10.3892/ol.2018.9376

miR‑339‑5p inhibits lung adenocarcinoma invasion and migration by directly targeting BCL6

Authors:
- Peng Li
- Huaqing Liu
- Yanmeng Li
- Yan Wang
- Lin Zhao
- Hui Wang
View Affiliations

Affiliations: Department of Respiratory Medicine, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China, The Third Department of Neurology, Zhangqiu People's Hospital, Jinan, Shandong 250200, P.R. China, Department of Laboratory Medicine, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China, Department of Public Health, Zhangqiu People's Hospital, Jinan, Shandong 250200, P.R. China, The Second Department of Respiratory Medicine, People's Hospital of Linyi, Linyi, Shandong 276003, P.R. China
Published online on: August 31, 2018 https://doi.org/10.3892/ol.2018.9376
Pages: 5785-5790
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Lung adenocarcinoma (LA) is a common non‑small cell lung cancer, but effective biomarkers to diagnose early LA are still lacking. Increasing evidence has indicated that the dysregulation of microRNAs (miRNAs) play crucial roles in LA progression. miR‑339‑5p has been recently confirmed to exert crucial functions in various cancers. Nevertheless, molecular mechanisms and effects of miR‑339‑5p on LA development still remain vague. In the present research, miR‑339‑5p expression was downregulated in human LA tissues. miR‑339‑5p overexpression in LA cells could remarkably suppress the LA cell invasion and migration. In addition, further studies indicated that miR‑339‑5p overexpression downregulated both the B‑cell lymphoma 6 (BCL6) mRNA and protein expressions by targeting the BCL6 3'‑UTR directly. Moreover, BCL6 knockdown could partially lessen the function of miR‑339‑5p in LA invasion and migration. In conclusion, the present data identified miR‑339‑5p as a novel LA suppressor which exerted its functions partly by negatively regulating BCL6.

View Figures

View References

1	Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI
2	Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI
3	Nana-Sinkam SP and Powell CA: Molecular biology of lung cancer: Diagnosis and management of lung cancer, 3rd ed: American College of Chest Physicians evidence-based clinical practice guidelines. Chest. 143 5 Suppl:e30S–e39S. 2013. View Article : Google Scholar : PubMed/NCBI
4	Bhargava A, Bunkar N, Aglawe A, Pandey KC, Tiwari R, Chaudhury K, Goryacheva IY and Mishra PK: Epigenetic biomarkers for risk assessment of particulate matter associated lung cancer. Curr Drug Targets. 19:1127–1147. 2018. View Article : Google Scholar : PubMed/NCBI
5	Peng Z, Pan L, Niu Z, Li W, Dang X, Wan L, Zhang R and Yang S: Identification of microRNAs as potential biomarkers for lung adenocarcinoma using integrating genomics analysis. Oncotarget. 8:64143–64156. 2017. View Article : Google Scholar : PubMed/NCBI
6	Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI
7	Nohata N, Hanazawa T, Kinoshita T, Okamoto Y and Seki N: MicroRNAs function as tumor suppressors or oncogenes: Aberrant expression of microRNAs in head and neck squamous cell carcinoma. Auris Nasus Larynx. 40:143–149. 2013. View Article : Google Scholar : PubMed/NCBI
8	Yang L, Liang H, Wang Y, Gao S, Yin K, Liu Z, Zheng X, Lv Y, Wang L, Zhang CY, et al: MiRNA-203 suppresses tumor cell proliferation, migration and invasion by targeting Slug in gastric cancer. Protein Cell. 7:383–387. 2016. View Article : Google Scholar : PubMed/NCBI
9	Feng C, Sun P, Hu J, Feng H, Li M, Liu G, Pan Y, Feng Y, Xu Y, Feng K, et al: miRNA-556-3p promotes human bladder cancer proliferation, migration and invasion by negatively regulating DAB2IP expression. Int J Oncol. 50:2101–2112. 2017. View Article : Google Scholar : PubMed/NCBI
10	Hu X, Zhang M, Miao J, Wang X and Huang C: miRNA-4317 suppresses human gastric cancer cell proliferation by targeting ZNF322. Cell Biol Int. Sep 7–2017.(Epub ahead of print).
11	Li Y, Zhang H, Dong Y, Fan Y, Li Y, Zhao C, Wang C, Liu J, Li X, Dong M, et al: MiR-146b-5p functions as a suppressor miRNA and prognosis predictor in non-small cell lung cancer. J Cancer. 8:1704–1716. 2017. View Article : Google Scholar : PubMed/NCBI
12	Wang D, Cao Q, Qu M, Xiao Z, Zhang M and Di S: MicroRNA-616 promotes the growth and metastasis of non-small cell lung cancer by targeting SOX7. Oncol Rep. 38:2078–2086. 2017. View Article : Google Scholar : PubMed/NCBI
13	Green MR, Vicente-Dueñas C, Alizadeh AA and Sánchez-García I: Hit-and-run lymphomagenesis by the Bcl6 oncogene. Cell Cycle. 13:1831–1832. 2014. View Article : Google Scholar : PubMed/NCBI
14	Kojima S, Hatano M, Okada S, Fukuda T, Toyama Y, Yuasa S, Ito H and Tokuhisa T: Testicular germ cell apoptosis in Bcl6-deficient mice. Development. 128:57–65. 2001.PubMed/NCBI
15	Nola S, Sin S, Bonin F, Lidereau R and Driouch K: A methodological approach to unravel organ-specific breast cancer metastasis. J Mammary Gland Biol Neoplasia. 17:135–145. 2012. View Article : Google Scholar : PubMed/NCBI
16	Yan H, Zhao M, Huang S, Chen P, Wu WY, Huang J, Wu ZS and Wu Q: Prolactin inhibits BCL6 expression in breast cancer cells through a microRNA-339-5p-dependent pathway. J Breast Cancer. 19:26–33. 2016. View Article : Google Scholar : PubMed/NCBI
17	Kamada Y, Sakai N, Sogabe S, Ida K, Oki H, Sakamoto K, Lane W, Snell G, Iida M, Imaeda Y, et al: Discovery of a B-cell lymphoma 6 protein-protein interaction inhibitor by a biophysics-driven fragment-based approach. J Med Chem. 60:4358–4368. 2017. View Article : Google Scholar : PubMed/NCBI
18	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
19	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
20	Rivera MP: Lung cancer in women: Differences in epidemiology, biology, histology, and treatment outcomes. Semin Respir Crit Care Med. 34:792–801. 2013. View Article : Google Scholar : PubMed/NCBI
21	Wang Y, Han R, Chen Z, Fu M, Kang J, Li K, Li L, Chen H and He Y: A transcriptional miRNA-gene network associated with lung adenocarcinoma metastasis based on the TCGA database. Oncol Rep. 35:2257–2269. 2016. View Article : Google Scholar : PubMed/NCBI
22	Wang YL, Chen CM, Wang XM and Wang L: Effects of miR-339-5p on invasion and prognosis of hepatocellular carcinoma. Clin Res Hepatol Gastroenterol. 40:51–56. 2016. View Article : Google Scholar : PubMed/NCBI
23	Wu ZS, Wu Q, Wang CQ, Wang XN, Wang Y, Zhao JJ, Mao SS, Zhang GH, Zhang N and Xu XC: MiR-339-5p inhibits breast cancer cell migration and invasion in vitro and may be a potential biomarker for breast cancer prognosis. BMC Cancer. 10:5422010. View Article : Google Scholar : PubMed/NCBI
24	Zhou C, Liu G, Wang L, Lu Y, Yuan L, Zheng L, Chen F, Peng F and Li X: MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1. PLoS One. 8:e631422013. View Article : Google Scholar : PubMed/NCBI
25	Jansson MD, Damas ND, Lees M, Jacobsen A and Lund AH: miR-339-5p regulates the p53 tumor-suppressor pathway by targeting MDM2. Oncogene. 34:1908–1918. 2015. View Article : Google Scholar : PubMed/NCBI
26	Xu L, Chen Y, Dutra-Clarke M, Mayakonda A, Hazawa M, Savinoff SE, Doan N, Said JW, Yong WH, Watkins A, et al: BCL6 promotes glioma and serves as a therapeutic target. Proc Natl Acad Sci USA. 114:3981–3986. 2017. View Article : Google Scholar : PubMed/NCBI
27	Zhu L, Feng H, Jin S, Tan M, Gao S, Zhuang H, Hu Z, Wang H, Song Z and Lin B: High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer. Tumour Biol. 39:10104283177116552017.doi: 10.1177/1010428317711655. View Article : Google Scholar : PubMed/NCBI
28	Zhu Z, Wang S, Zhu J, Yang Q, Dong H and Huang J: MicroRNA-544 down-regulates both Bcl6 and Stat3 to inhibit tumor growth of human triple negative breast cancer. Biol Chem. 397:1087–1095. 2016. View Article : Google Scholar : PubMed/NCBI