miR-145-5p inhibits the proliferation and migration of bladder cancer cells by targeting TAGLN2

Zhang,Haijian; Jiang,Meijuan; Liu,Qingjun; Han,Zhixing; Zhao,Yuqian; Ji,Shiqi

doi:10.3892/ol.2018.9436

miR-145-5p inhibits the proliferation and migration of bladder cancer cells by targeting TAGLN2

Authors:
- Haijian Zhang
- Meijuan Jiang
- Qingjun Liu
- Zhixing Han
- Yuqian Zhao
- Shiqi Ji
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Affiliations: Department of Urology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China, Department of Neurology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China
Published online on: September 12, 2018 https://doi.org/10.3892/ol.2018.9436
Pages: 6355-6360
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA-145-5p (miR-145-5p) is found to be involved in tumor development and progression. However, there are few studies on the effects of miR-145-5p on bladder cancer (BC). The role of miR-145-5p in BC was predicted by analysis of cell proliferation and migration in this study. The miR-145-5p and transgelin-2 (TAGLN2) expressions were evaluated via reverse transcription-quantitative PCR (RT-qPCR) or western blot analysis. The MTT and Transwell assay assessed cell proliferation and migration. TAGLN2 targeted to miR-145-5p was determined using luciferase assays. The results showed that the miR-145-5p downregulation was found in BC. miR-145-5p overexpression inhibited cell proliferation and migration in BC. Moreover, miR-145-5p directly targeted TAGLN2, and TAGLN2 expression was increased in BC. In addition, the high expression of TAGLN2 promoted cell proliferation and migration in BC. miR-145-5p appeared to regulate TAGLN2 in BC, and it also inhibited the cell proliferation and migration. The novel miR-145-5p/TAGLN2 axis may provide new therapeutic implications for BC.

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