Open Access

miR‑491‑5p inhibits osteosarcoma cell proliferation by targeting PKM2

  • Authors:
    • Ting Chen
    • Yuyang Li
    • Wenliang Cao
    • Yadong Liu
  • View Affiliations

  • Published online on: September 18, 2018     https://doi.org/10.3892/ol.2018.9451
  • Pages: 6472-6478
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence has indicated that microRNAs (miRNAs/miRs) are associated with tumorigenesis and the development of numerous cancer types. Previous studies have suggested miRNA‑491‑5p is downregulated in osteosarcoma (OS) and functions as a tumor suppressor. However, the biological roles and underlying mechanisms associated with miR‑491‑5p function in OS require further exploration. In the present study, it was demonstrated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) that miR‑491‑5p was downregulated in 36 pairs of OS tissues, compared with in adjacent normal bone tissues. Furthermore, CCK‑8 and colony formation assays indicated that miR‑491‑5p mimics suppressed OS cell proliferation. However, an miR‑491‑5p inhibitor enhanced cell proliferation. In addition, luciferase reporter assays, RT‑qPCR and western blot analysis demonstrated that PKM2 was a direct target of miR‑491‑5p. The miR‑491‑5p mimic inhibited the mRNA and protein expression of PKM2, while the miR‑491‑5p inhibitor promoted PKM2 mRNA and protein expression. In addition, PKM2 overexpression reversed the proliferation‑inhibiting effects of miR‑491‑5p in OS cells. Therefore, these results indicated that miR‑491‑5p serves as a tumor suppressor in OS cells, which may be important in OS treatment.
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November-2018
Volume 16 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Chen T, Li Y, Cao W and Liu Y: miR‑491‑5p inhibits osteosarcoma cell proliferation by targeting PKM2. Oncol Lett 16: 6472-6478, 2018.
APA
Chen, T., Li, Y., Cao, W., & Liu, Y. (2018). miR‑491‑5p inhibits osteosarcoma cell proliferation by targeting PKM2. Oncology Letters, 16, 6472-6478. https://doi.org/10.3892/ol.2018.9451
MLA
Chen, T., Li, Y., Cao, W., Liu, Y."miR‑491‑5p inhibits osteosarcoma cell proliferation by targeting PKM2". Oncology Letters 16.5 (2018): 6472-6478.
Chicago
Chen, T., Li, Y., Cao, W., Liu, Y."miR‑491‑5p inhibits osteosarcoma cell proliferation by targeting PKM2". Oncology Letters 16, no. 5 (2018): 6472-6478. https://doi.org/10.3892/ol.2018.9451