Open Access

Apoptosis of mouse myeloma cells induced by curcumin via the Notch3‑p53 signaling axis

  • Authors:
    • Ying Zhang
    • Xin‑Yu Lin
    • Jiao‑Hui Zhang
    • Zheng‑Lu Xie
    • Hui Deng
    • Yi‑Fang Huang
    • Xiao‑Hong Huang
  • View Affiliations

  • Published online on: October 17, 2018     https://doi.org/10.3892/ol.2018.9591
  • Pages: 127-134
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Resistance to apoptosis is a characteristic of cancer. Curcumin has become a potential anticancer drug for its pro‑apoptotic effects, but the underlying mechanisms remain unclear. Furthermore, the Notch3‑p53 signaling axis serves an important role in cell fate. The present study was designed to investigate the antitumor effect of curcumin by the Notch3‑p53 axis in mouse myeloma P3X63Ag8 cells. The effects of curcumin on the viability of P3X63Ag8 cells were evaluated using an MTT assay. Quantitative expression of the Notch3‑p53 signaling axis‑associated genes was measured by reverse transcription‑quantitative polymerase chain reaction, and western blot analysis was used to investigate the expression of proteins. Additionally, flow cytometry was used to measure the ratio of apoptosis. The results demonstrated that curcumin could significantly inhibit cell viability. No significant pro‑apoptotic effect was observed when the concentration of curcumin was <30 µM. At 30 µM, curcumin‑treated cells exhibited an apoptotic phenomenon, and the ratio of late apoptosis increased with the concentration of curcumin, and reached 28.4 and 51.8% in the medium‑ and high‑dose groups, respectively. Curcumin inhibited the expression of Notch3, while the middle‑ and high‑dose groups promoted p53. The expression of Notch3‑responsive genes Hes family BHLH transcription factor 1 and Hes‑related family transcription factor with YRPW motif 1 were notably promoted. Curcumin treatment significantly downregulated B‑cell lymphoma‑2 (Bcl‑2) at the mRNA and protein levels, but upregulated Bcl‑2‑associated X. These data indicated that curcumin exhibited antitumor effects in mouse myeloma cells with induction of apoptosis by affecting the Notch3‑p53 signaling axis.
View Figures
View References

Related Articles

Journal Cover

January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Lin XY, Zhang JH, Xie ZL, Deng H, Huang YF and Huang XH: Apoptosis of mouse myeloma cells induced by curcumin via the Notch3‑p53 signaling axis. Oncol Lett 17: 127-134, 2019.
APA
Zhang, Y., Lin, X., Zhang, J., Xie, Z., Deng, H., Huang, Y., & Huang, X. (2019). Apoptosis of mouse myeloma cells induced by curcumin via the Notch3‑p53 signaling axis. Oncology Letters, 17, 127-134. https://doi.org/10.3892/ol.2018.9591
MLA
Zhang, Y., Lin, X., Zhang, J., Xie, Z., Deng, H., Huang, Y., Huang, X."Apoptosis of mouse myeloma cells induced by curcumin via the Notch3‑p53 signaling axis". Oncology Letters 17.1 (2019): 127-134.
Chicago
Zhang, Y., Lin, X., Zhang, J., Xie, Z., Deng, H., Huang, Y., Huang, X."Apoptosis of mouse myeloma cells induced by curcumin via the Notch3‑p53 signaling axis". Oncology Letters 17, no. 1 (2019): 127-134. https://doi.org/10.3892/ol.2018.9591