Upregulation of TMEFF2 is involved in the antiproliferative effects of vitamin C and tyrphostin AG490 on GES‑1 and AGS cells

  • Authors:
    • Hongchao Han
    • Jie Xu
    • Weiwei Ji
    • Lisheng Wang
    • Aikun Wang
  • View Affiliations

  • Published online on: October 25, 2018     https://doi.org/10.3892/ol.2018.9619
  • Pages: 652-659
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Abstract

Transmembrane protein with epidermal growth factor (EGF)‑like and two follistatin motifs 2 (TMEFF2) is downregulated in human gastric cancer, and its levels are associated with tumor aggressiveness. Herein, a positive correlation was identified between serum vitamin C levels (µg/ml) and mRNA levels of TMEFF2 in gastric cancer tissue. TMEFF2 silencing promotes cell proliferation in GES‑1 normal human gastric epithelial cells and AGS human gastric adenocarcinoma cells. Notably, vitamin C and AG490 exerted antiproliferative effects on the two cell lines. The present study demonstrated that small interfering (si)‑RNA‑TMEFF2 exerts pro‑proliferative effects on GES‑1 and AGS cells. The results revealed that vitamin C significantly inhibited the growth of GES‑1 and AGS cells by reducing cell viability, decreasing the expression of proliferating cell nuclear antigen (PCNA), and blocking the STAT3 pathway. Moreover, siRNA‑TMEFF2‑induced enhanced cell viability and PCNA expression were significantly reversed by additional vitamin C treatment; notably, markedly enhanced TMEFF2 expression was observed. Upregulated TMEFF2 expression was observed in association with the antiproliferative effect of AG490. In conclusion, serum vitamin C content (µg/ml) was positively correlated with the mRNA levels of TMEFF2 in gastric cancer tissue. Exploring novel drugs that target TMEFF2 is a potential therapeutic strategy for blocking human GC.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Han H, Xu J, Ji W, Wang L and Wang A: Upregulation of TMEFF2 is involved in the antiproliferative effects of vitamin C and tyrphostin AG490 on GES‑1 and AGS cells. Oncol Lett 17: 652-659, 2019.
APA
Han, H., Xu, J., Ji, W., Wang, L., & Wang, A. (2019). Upregulation of TMEFF2 is involved in the antiproliferative effects of vitamin C and tyrphostin AG490 on GES‑1 and AGS cells. Oncology Letters, 17, 652-659. https://doi.org/10.3892/ol.2018.9619
MLA
Han, H., Xu, J., Ji, W., Wang, L., Wang, A."Upregulation of TMEFF2 is involved in the antiproliferative effects of vitamin C and tyrphostin AG490 on GES‑1 and AGS cells". Oncology Letters 17.1 (2019): 652-659.
Chicago
Han, H., Xu, J., Ji, W., Wang, L., Wang, A."Upregulation of TMEFF2 is involved in the antiproliferative effects of vitamin C and tyrphostin AG490 on GES‑1 and AGS cells". Oncology Letters 17, no. 1 (2019): 652-659. https://doi.org/10.3892/ol.2018.9619