MicroRNA-153 suppresses cell invasion by targeting SNAI1 and predicts patient prognosis in glioma

Zhao,Wei; Yin,Chang-You; Jiang,Jing; Kong,Wei; Xu,Hao; Zhang,Hongtao

doi:10.3892/ol.2018.9706

MicroRNA-153 suppresses cell invasion by targeting SNAI1 and predicts patient prognosis in glioma

Authors:
- Wei Zhao
- Chang-You Yin
- Jing Jiang
- Wei Kong
- Hao Xu
- Hongtao Zhang
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Affiliations: Department of Neurosurgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China, Department of Emergency, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China
Published online on: November 15, 2018 https://doi.org/10.3892/ol.2018.9706
Pages: 1189-1195
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioma is the most common and rapidly progressive type of malignant primary brain tumor in adults. miR‑153 plays a major role in many malignancies; nevertheless, few studies have been conducted on glioma. The aim of the present study was to explore the role of miR‑153 and SNAI1 on invasion in glioma. Reverse transcription-quantitative PCR was employed to measure the expression levels of miR‑153 and SNAI1 mRNA. Transwell assay was utilized to calculate the capacity of invasion. Luciferase report assay was applied to detect whether SNAI1 was a target of miR‑153. miR‑153 was downregulated in glioma tissues and cells versus paracancerous tissues and normal immortalized gliocyte HEB cells. Transwell assay was used to measure whether a low expression of miR‑153 in glioma indicated inhibition of cell invasion. We verified that SNAI1 was a target of miR‑153 and had a negative association with miR‑153 detected by luciferase reporter assay. Additionally, miR‑153 suppressed cell invasive ability by regulating SNAI1 expression, whose partial function was reversed by SNAI1. miR‑153 suppressed cell invasion of glioma by directly targeting SNAI1. Thus, miR‑153/SNAI1 axis may be a novel target for cervical cancer treatment.

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1	Westphal M and Lamszus K: The neurobiology of gliomas: From cell biology to the development of therapeutic approaches. Nat Rev Neurosci. 12:495–508. 2011. View Article : Google Scholar : PubMed/NCBI
2	Kwiatkowski SC, Guerrero PA, Hirota S, Chen Z, Morales JE, Aghi M and McCarty JH: Neuropilin-1 modulates TGFβ signaling to drive glioblastoma growth and recurrence after anti-angiogenic therapy. PLoS One. 12:e01850652017. View Article : Google Scholar : PubMed/NCBI
3	Louis DN: Molecular pathology of malignant gliomas. Annu Rev Pathol. 1:97–117. 2006. View Article : Google Scholar : PubMed/NCBI
4	Sayegh ET, Kaur G, Bloch O and Parsa AT: Systematic review of protein biomarkers of invasive behavior in glioblastoma. Mol Neurobiol. 49:1212–1244. 2014. View Article : Google Scholar : PubMed/NCBI
5	Munthe S, Halle B, Boldt HB, Christiansen H, Schmidt S, Kaimal V, Xu J, Zabludoff S, Mollenhauer J, Poulsen FR, et al: Shift of microRNA profile upon glioma cell migration using patient-derived spheroids and serum-free conditions. J Neurooncol. 132:45–54. 2017. View Article : Google Scholar : PubMed/NCBI
6	Siomi H and Siomi MC: Posttranscriptional regulation of microRNA biogenesis in animals. Mol Cell. 38:323–332. 2010. View Article : Google Scholar : PubMed/NCBI
7	Ha M and Kim VN: Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol. 15:509–524. 2014. View Article : Google Scholar : PubMed/NCBI
8	Fabbri M: MicroRNAs and cancer: Towards a personalized medicine. Curr Mol Med. 13:751–756. 2013. View Article : Google Scholar : PubMed/NCBI
9	Gonzalez-Duarte RJ, Cazares-Ordonez V and Avila-Chavez E: The microRNA biogenesis machinery: Regulation by steroid hormones and alterations in cancer. Rev Invest Clin. 66:460–464. 2014.PubMed/NCBI
10	Zhang Z, Sun J, Bai Z, Li H, He S, Chen R and Che X: MicroRNA-153 acts as a prognostic marker in gastric cancer and its role in cell migration and invasion. Onco Targets Ther. 8:357–364. 2015.PubMed/NCBI
11	Wang W, Peng B, Wang D, Ma X, Jiang D, Zhao J and Yu L: Human tumor microRNA signatures derived from large-scale oligonucleotide microarray datasets. Int J Cancer. 129:1624–1634. 2011. View Article : Google Scholar : PubMed/NCBI
12	Zuo J, Wang D, Shen H, Liu F, Han J and Zhang X: MicroRNA-153 inhibits tumor progression in esophageal squamous cell carcinoma by targeting SNAI1. Tumour Biol. 37:16135–16140. 2016. View Article : Google Scholar
13	Wu X, Li L, Li Y and Liu Z: miR-153 promotes breast cancer cell apoptosis by targeting HECTD3. Am J Cancer Res. 6:1563–1571. 2016.PubMed/NCBI
14	Bai Z, Sun J, Wang X, Wang H, Pei H and Zhang Z: MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma. Oncol Rep. 34:595–602. 2015. View Article : Google Scholar : PubMed/NCBI
15	Shan N, Shen L, Wang J, He D and Duan C: miR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19. Biochem Biophys Res Commun. 456:385–391. 2015. View Article : Google Scholar : PubMed/NCBI
16	Zeng HF, Yan S and Wu SF: MicroRNA-153-3p suppress cell proliferation and invasion by targeting SNAI1 in melanoma. Biochem Biophys Res Commun. 487:140–145. 2017. View Article : Google Scholar : PubMed/NCBI
17	Zhang L, Pickard K, Jenei V, Bullock MD, Bruce A, Mitter R, Kelly G, Paraskeva C, Strefford J, Primrose J, et al: miR-153 supports colorectal cancer progression via pleiotropic effects that enhance invasion and chemotherapeutic resistance. Cancer Res. 73:6435–6447. 2013. View Article : Google Scholar : PubMed/NCBI
18	Nieto MA: The snail superfamily of zinc-finger transcription factors. Nat Rev Mol Cell Biol. 3:155–166. 2002. View Article : Google Scholar : PubMed/NCBI
19	Peinado H, Ballestar E, Esteller M and Cano A: Snail mediates E-cadherin repression by the recruitment of the Sin3A/histone deacetylase 1 (HDAC1)/HDAC2 complex. Mol Cell Biol. 24:306–319. 2004. View Article : Google Scholar : PubMed/NCBI
20	Natsugoe S, Uchikado Y, Okumura H, Matsumoto M, Setoyama T, Tamotsu K, Kita Y, Sakamoto A, Owaki T, Ishigami S, et al: Snail plays a key role in E-cadherin-preserved esophageal squamous cell carcinoma. Oncol Rep. 17:517–523. 2007.PubMed/NCBI
21	Dong Q, Cai N, Tao T, Zhang R, Yan W, Li R, Zhang J, Luo H, Shi Y, Luan W, et al: An axis involving SNAI1, microRNA-128 and SP1 modulates glioma progression. PLoS One. 9:e986512014. View Article : Google Scholar : PubMed/NCBI
22	Allen M, Bjerke M, Edlund H, Nelander S and Westermark B: Origin of the U87MG glioma cell line: Good news and bad news. Sci Transl Med. 8:54re32016. View Article : Google Scholar
23	Livak KJ and Scmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
24	Cui Y, Zhao J, Yi L and Jiang Y: microRNA-153 targets mTORC2 component rictor to inhibit glioma cells. PLoS One. 11:e01569152016. View Article : Google Scholar : PubMed/NCBI
25	Christodoulatos GS and Dalamaga M: Micro-RNAs as clinical biomarkers and therapeutic targets in breast cancer: Quo vadis? World J Clin Oncol. 5:71–81. 2014. View Article : Google Scholar : PubMed/NCBI
26	Lauressergues D, Couzigou JM, Clemente HS, Martinez Y, Dunand C, Bécard G and Combier JP: Primary transcripts of microRNAs encode regulatory peptides. Nature. 520:90–93. 2015. View Article : Google Scholar : PubMed/NCBI
27	Shukla GC, Singh J and Barik S: MicroRNAs: Processing, maturation, target recognition and regulatory functions. Mol Cell Pharmacol. 3:83–92. 2011.PubMed/NCBI
28	Esquela-Kerscher A and Slack FJ: Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer. 6:259–269. 2006. View Article : Google Scholar : PubMed/NCBI
29	Niu G, Li B, Sun L and An C: MicroRNA-153 inhibits osteosarcoma cells proliferation and invasion by targeting TGF-β2. PLoS One. 10:e01192252015. View Article : Google Scholar : PubMed/NCBI