Association of the invasiveness of colon cancer with the expression of C/EBPα

Li,Wei; Jiang,Liang‑Jun; Zhou,Xiao‑Jun; Lu,Xian‑Zhou; Liu,Long‑Fei; Wang,Song

doi:10.3892/ol.2018.9755

Association of the invasiveness of colon cancer with the expression of C/EBPα

Authors:
- Wei Li
- Liang‑Jun Jiang
- Xiao‑Jun Zhou
- Xian‑Zhou Lu
- Long‑Fei Liu
- Song Wang
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Affiliations: Department of General Surgery, Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421002, P.R. China, Department of Gastroenterology, Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421002, P.R. China
Published online on: November 23, 2018 https://doi.org/10.3892/ol.2018.9755
Pages: 1974-1979

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Abstract

The present study aimed to investigate the association of the invasiveness of colon cancer (CC) with the expression of CCAAT/enhancer binding protein α (C/EBPα). Immunohistochemistry was performed to determine the expression of C/EBPα in the cancer and adjacent tissue samples from 48 patients with CC. A pCDGFP‑C/EBPα eukaryotic expression vector was constructed, and a wound‑healing assay was performed to observe the effect of transfection on the migration of SW480 cells. In addition, the expression levels of tumor invasion‑associated proteins, including Kruppel‑like factor 5 (KLF5), matrix metallopeptidase (MMP)‑2, MMP‑9, and E‑cadherin (ECD) were detected subsequent to transfection. Immunohistochemistry analysis demonstrated that the rate of low C/EBPα expression in normal tissue was 6.25%, whereas the rate in CC tissues was 68.75%; this difference was statistically significant (P<0.05). The patients with lower C/EBPα expression exhibited statistically larger tumor diameters, more advanced tumor‑node‑metastasis (TMN) stages and a greater likelihood of lymph node metastasis. The overexpression of C/EBPα significantly reduced the mobility of SW480 cells, and the expression of KLF5, MMP‑2 and MMP‑9 was reduced, whereas the expression of ECD was increased. In conclusion, C/EBPα was downregulated in CC tissue samples, and associated with the TMN stage and metastasis of CC; in addition, the overexpression of C/EBPα significantly reduced the invasiveness of CC cells. This may be significant for the diagnosis and treatment of CC in the future.

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