miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5

Wang,Jian; Chu,Yanfeng; Xu,Mei; Zhang,Xiumei; Zhou,Yumei; Xu,Mei

doi:10.3892/ol.2018.9843

miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5

Authors:
- Jian Wang
- Yanfeng Chu
- Mei Xu
- Xiumei Zhang
- Yumei Zhou
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Affiliations: The First Department of Digestive Medicine, People's Hospital of Linyi, Linyi, Shandong 276000, P.R. China, Deparment of Clinical Laboratory, Yantaishan Hospital, Yantai, Shandong 264001, P.R. China, Department of General Surgery, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China, Department of Internal Medicine, People's Hospital of Zhangqiu, Jinan, Shandong 250200, P.R. China, Department of Oncology, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China, Department of Obstetrics, People's Hospital of Chengyang, Qingdao, Shandong 266001, P.R. China
Published online on: December 18, 2018 https://doi.org/10.3892/ol.2018.9843
Pages: 2221-2227
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have reported that microRNAs regulate gene expression and transcription. miR-21 have been identified to play a role in many types of cancer. KLF5 functions as a tumor inhibitor in certain cancers. However, the role of KLF5 plays in hepatocellular carcinoma (HCC), especially concerning the relationship between miR-21 and the KLF5 gene remains to be determined. Reverse transcription-quantitative PCR (RT-qPCR), western blot analysis, as well as luciferase reporter and Transwell assays were used to determine the expression of miR-21 and KLF5 in Huh 7, SK-HEP-1, LO-2, and HCC tissues. In HCC cells and tissues, the upregulation of miR-21 was identified. HCC cell migratory and invasive abilities significantly increased because of miR-21 overexpression. KLF5 expression was inhibited by miR-21 by targeting its 3'-UTR. KLF5 overexpression alleviated the effect induced by miR-21 on the migratory and invasive ability of the Huh 7 cells. The results therefore show that, HCC cell migration and invasion is significantly suppressed by miR-21 via targeting KLF5. The newly identified miR-21/KLF5 axis provides a useful therapeutic biomarker for HCC treatment.

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