lncRNA‑NEF is downregulated in osteosarcoma and inhibits cancer cell migration and invasion by downregulating miRNA‑21

Yang,Qinglei; Yu,Hongying; Yin,Qingshui; Hu,Xiaoming; Zhang,Chengcheng

doi:10.3892/ol.2019.10276

June-2019 Volume 17 Issue 6

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June-2019 Volume 17 Issue 6

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lncRNA‑NEF is downregulated in osteosarcoma and inhibits cancer cell migration and invasion by downregulating miRNA‑21

Authors:
- Qinglei Yang
- Hongying Yu
- Qingshui Yin
- Xiaoming Hu
- Chengcheng Zhang
View Affiliations / Copyright

Affiliations: Department of Orthopedics, Guangzhou School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Pharmacy, Yuebei People's Hospital, Shaoguan, Guangdong 512000, P.R. China

Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5403-5408
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Published online on: April 18, 2019

https://doi.org/10.3892/ol.2019.10276
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Abstract

Long non‑coding RNA (lncRNA)‑NEF inhibits cancer metastasis in hepatocellular carcinoma; however, its role in other malignancies is unknown. The present study revealed that plasma lncRNA‑NEF was downregulated, while miRNA‑21 was upregulated in patients with osteosarcoma compared with healthy controls. Plasma lncRNA‑NEF and miRNA‑21 were negatively correlated in patients with osteosarcoma and healthy controls. Downregulation of lncRNA‑NEF and upregulation of miRNA‑21 distinguished patients with osteosarcoma from healthy controls. lncRNA‑NEF overexpression mediated the inhibition of miRNA‑21 expression in osteosarcoma cell lines, while miRNA‑21 overexpression did not significantly affect the expression of lncRNA‑NEF. lncRNA‑NEF overexpression inhibited, while miRNA‑21 overexpression promoted, migration and invasion of osteosarcoma cell lines in vitro. miRNA‑21 overexpression partially compensated the inhibitory effects of lncRNA‑NEF overexpression on osteosarcoma cell migration and invasion. Therefore, lncRNA‑NEF may inhibit cancer cell migration and invasion in osteosarcoma by downregulating miRNA‑21.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

lncRNA‑NEF is downregulated in osteosarcoma and inhibits cancer cell migration and invasion by downregulating miRNA‑21

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