Combination of quercetin and Adriamycin effectively suppresses the growth of refractory acute leukemia

  • Authors:
    • Yingxu Shi
    • Xiaotian Su
    • Hongwei Cui
    • Lei Yu
    • Hua Du
    • Yanqiu Han
  • View Affiliations

  • Published online on: May 2, 2019     https://doi.org/10.3892/ol.2019.10299
  • Pages: 153-160
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to investigate the effect of combined treatment with quercetin and Adriamycin (doxorubicin) on the development of refractory acute leukemia. Primary leukemic cells were isolated from patients with refractory drug‑resistant acute leukemia. The Cell Counting Kit‑8 assay was used to detect the proliferation of cells treated with a range of doses of Adriamycin, quercetin and a combination of the two drugs. Non‑irradiated mice were used to establish a T cell acute lymphoblastic leukemia (T‑ALL) model, which was subsequently treated with Adriamycin, quercetin and a combination of the two drugs. The survival time was recorded, and white and red blood cells and platelets in mouse peripheral blood were counted. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of cardiac tissues were measured as indicators of oxidative stress and damage. Proliferation of primary leukemic cells was reduced by Adriamycin depending on the dose (0.06, 0.6 or 6 µg/ml) and treatment duration (24, 48 or 72 h) compared with the vehicle treated group. Co‑treatment with quercetin achieved a similar suppression of leukemic cell proliferation when a lower dose of Adriamycin (0.03, 0.3 or 3 µg/ml) was administered for the same duration. The survival of non‑irradiated mice with T‑ALL was improved by co‑treatment with a high dose of Adriamycin and quercetin compared with either treatment alone. Compared with treatment with Adriamycin alone, the combined treatment with Adriamycin and quercetin significantly enhanced the SOD activity and reduced the MDA content in the heart. Therefore, quercetin may enhance the effects of Adriamycin on refractory acute leukemia.
View Figures
View References

Related Articles

Journal Cover

July-2019
Volume 18 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Shi Y, Su X, Cui H, Yu L, Du H and Han Y: Combination of quercetin and Adriamycin effectively suppresses the growth of refractory acute leukemia. Oncol Lett 18: 153-160, 2019.
APA
Shi, Y., Su, X., Cui, H., Yu, L., Du, H., & Han, Y. (2019). Combination of quercetin and Adriamycin effectively suppresses the growth of refractory acute leukemia. Oncology Letters, 18, 153-160. https://doi.org/10.3892/ol.2019.10299
MLA
Shi, Y., Su, X., Cui, H., Yu, L., Du, H., Han, Y."Combination of quercetin and Adriamycin effectively suppresses the growth of refractory acute leukemia". Oncology Letters 18.1 (2019): 153-160.
Chicago
Shi, Y., Su, X., Cui, H., Yu, L., Du, H., Han, Y."Combination of quercetin and Adriamycin effectively suppresses the growth of refractory acute leukemia". Oncology Letters 18, no. 1 (2019): 153-160. https://doi.org/10.3892/ol.2019.10299