Open Access

Long non‑coding RNA HAND2‑AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma

  • Authors:
    • Shunguang Chen
    • Xiaoming Xu
    • Shengjun Lu
    • Biao Hu
  • View Affiliations

  • Published online on: June 7, 2019     https://doi.org/10.3892/ol.2019.10445
  • Pages: 1323-1329
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNA heart and neural crest derivatives expressed 2‑antisense RNA 1 (lncRNA HAND2‑AS1) is a known tumor suppressor gene in endometrioid endometrial carcinoma; however, its function in osteosarcoma is currently unknown. In the present study, HAND2‑AS1 expression in the tumor tissues and adjacent healthy tissues of patients with osteosarcoma, and in the serum of patients and heathy controls was detected by reverse transcription‑quantitative polymerase chain reaction. lncRNA HAND2‑AS1 small interfering RNA was transfected into osteosarcoma cells, and cell proliferation, glucose transporter 1 (GLUT1) expression and glucose uptake were detected using the Cell Counting Kit‑8, western blotting and glucose uptake assays, respectively. The results revealed that the expression levels of HAND2‑AS1 were reduced in cancer tissues compared with those in healthy tissues. Levels of HAND2‑AS1 were also reduced in the serum of patients with osteosarcoma compared with those of the control subjects. A significant association was observed between serum levels of HAND2‑AS1 and tumor size, but not tumor metastasis. HAND2‑AS1‑knockdown promoted osteosarcoma cell proliferation, increased glucose uptake and upregulated GLUT1 expression. It was therefore concluded that lncRNA HAND2‑AS1 may inhibit the proliferation of osteosarcoma cells by targeting glucose metabolism.
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August-2019
Volume 18 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen S, Xu X, Lu S and Hu B: Long non‑coding RNA HAND2‑AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma. Oncol Lett 18: 1323-1329, 2019.
APA
Chen, S., Xu, X., Lu, S., & Hu, B. (2019). Long non‑coding RNA HAND2‑AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma. Oncology Letters, 18, 1323-1329. https://doi.org/10.3892/ol.2019.10445
MLA
Chen, S., Xu, X., Lu, S., Hu, B."Long non‑coding RNA HAND2‑AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma". Oncology Letters 18.2 (2019): 1323-1329.
Chicago
Chen, S., Xu, X., Lu, S., Hu, B."Long non‑coding RNA HAND2‑AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma". Oncology Letters 18, no. 2 (2019): 1323-1329. https://doi.org/10.3892/ol.2019.10445