Correlation of LAMA3 with onset and prognosis of ovarian cancer

Tang,Lin; Wang,Pin; Wang,Qilin; Zhong,Lan

doi:10.3892/ol.2019.10600

Correlation of LAMA3 with onset and prognosis of ovarian cancer

Authors:
- Lin Tang
- Pin Wang
- Qilin Wang
- Lan Zhong
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Affiliations: Emergency Obstetrics and Gynecology Department, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: July 10, 2019 https://doi.org/10.3892/ol.2019.10600
Pages: 2813-2818
Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Correlation of laminin subunit α3 (LAMA3) gene with onset and prognosis of ovarian cancer was investigated. In total, 210 ovarian cancer patients who received surgical resection in West China Second Hospital from March 2011 to March 2013 were randomly selected, and another 160 non‑ovarian cancer patients who needed ovariectomy were also selected. The relative expression of LAMA3 gene was compared via quantitative polymerase chain reaction (qPCR) in carcinoma tissues, para‑carcinoma tissues and non‑carcinoma normal tissues in ovarian cancer patients. The methylation level was compared among the above three tissue types. The correlation between the mutation site rs12373237 in LAMA3 gene and onset was analyzed. The expression of laminin in ovarian cancer was detected using immunohistochemistry. Moreover, the 5‑year survival rate after operation was recorded and the survival curve was plotted. The expression level of LAMA3 was lower in carcinoma tissues than those in normal tissues and para‑carcinoma tissues (P<0.05). The methylation degree was lower in para‑carcinoma tissues and normal tissues than that in carcinoma tissues (P<0.05). The CC homozygous mutation of rs12373237 was highly correlated with the onset of ovarian cancer (OR=4.333, P=0.028). The expression of LAMA3 was classified via immunohistochemistry, and the number in high‑expression group (63.8%) was larger than that in low‑expression group (36.2%) (P<0.05). According to the analysis of 5‑year survival rate, the recurrence‑free survival rate and overall survival rate in LAMA3 high‑expression group were significantly higher than those in LAMA3 low‑expression group (P<0.05). The expression level and base mutation of LAMA3 gene can change the level of laminin, which have a certain influence on the onset and prognosis of ovarian cancer.

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1	Xu Y, Cheng L, Dai H, Zhang R, Wang M, Shi T, Sun M, Cheng X and Wei Q: Variants in Notch signalling pathway genes, PSEN1 and MAML2, predict overall survival in Chinese patients with epithelial ovarian cancer. J Cell Mol Med. 22:4975–4984. 2018. View Article : Google Scholar : PubMed/NCBI
2	Oliveira-Ferrer L, Goswami R, Galatenko V, Ding Y, Eylmann K, Legler K, Kürti S, Schmalfeldt B and Milde-Langosch K: Prognostic impact of CEACAM1 in node-negative ovarian cancer patients. Dis Markers. 2018:67142872018. View Article : Google Scholar : PubMed/NCBI
3	Rhyasen GW, Yao Y, Zhang J, Dulak A, Castriotta L, Jacques K, Zhao W, Gharahdaghi F, Hattersley MM, Lyne PD, et al: BRD4 amplification facilitates an oncogenic gene expression program in high-grade serous ovarian cancer and confers sensitivity to BET inhibitors. PLoS One. 13:e02008262018. View Article : Google Scholar : PubMed/NCBI
4	Castro BG, Dos Reis R, Cintra GF, Sousa MM, Vieira MA and Andrade CE: Predictive factors for surgical morbidities and adjuvant chemotherapy delay for advanced ovarian cancer patients treated by primary debulking surgery or interval debulking surgery. Int J Gynecol Cancer. 28:1520–1528. 2018. View Article : Google Scholar : PubMed/NCBI
5	Saxena P, Severi L, Santucci M, Taddia L, Ferrari S, Luciani R, Marverti G, Marraccini C, Tondi D, Mor M, et al: Conformational propensity and biological studies of proline mutated LR peptides inhibiting human thymidylate synthase and ovarian cancer cell growth. J Med Chem. 61:7374–7380. 2018. View Article : Google Scholar : PubMed/NCBI
6	Shu C, Yan D, Mo Y, Gu J, Shah N and He J: Long noncoding RNA lncARSR promotes epithelial ovarian cancer cell proliferation and invasion by association with HuR and miR-200 family. Am J Cancer Res. 8:981–992. 2018.PubMed/NCBI
7	Coogan PF, Rosenberg L, Palmer JR, Strom BL, Zauber AG and Shapiro S: Statin use and the risk of breast and prostate cancer. Epidemiology. 13:262–267. 2002. View Article : Google Scholar : PubMed/NCBI
8	Chen P, Fang X, Xia B, Zhao Y, Li Q and Wu X: Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR-125b with MCL-1 by regulating mitochondrial apoptosis pathways in ovarian cancer. Cancer Med. 7:4530–4541. 2018. View Article : Google Scholar : PubMed/NCBI
9	Hong SS, Zhang MX, Zhang M, Yu Y, Chen J, Zhang XY and Xu CJ: Follicle-stimulating hormone peptide-conjugated nanoparticles for targeted shRNA delivery lead to effective gro-α silencing and antitumor activity against ovarian cancer. Drug Deliv. 25:576–584. 2018. View Article : Google Scholar : PubMed/NCBI
10	Ahmad F, Shah K, Umair M, Irfanullah JA, Khan S, Muhammad D, Basit S, Wakil SM, Ramzan K, et al: Novel autosomal recessive LAMA3 and PLEC variants underlie junctional epidermolysis bullosa generalized intermediate and epidermolysis bullosa simplex with muscular dystrophy in two consanguineous families. Clin Exp Dermatol. 43:752–755. 2018. View Article : Google Scholar : PubMed/NCBI
11	Zhang J, Wang H, Wang Y, Dong W, Jiang Z and Yang G: Substrate-mediated gene transduction of LAMA3 for promoting biological sealing between titanium surface and gingival epithelium. Colloids Surf B Biointerfaces. 161:314–323. 2018. View Article : Google Scholar : PubMed/NCBI
12	Reimer A, Schwieger-Briel A, He Y, Leppert J, Schauer F, Kiritsi D, Schneider H, Ott H, Bruckner-Tuderman L and Has C: Natural history and clinical outcome of junctional epidermolysis bullosa generalized intermediate due to a LAMA3 mutation. Br J Dermatol. 178:973–975. 2018. View Article : Google Scholar : PubMed/NCBI
13	Gostyńska KB, Yan Yuen W, Pasmooij AM, Stellingsma C, Pas HH, Lemmink H and Jonkman MF: Carriers with functional null mutations in LAMA3 have localized enamel abnormalities due to haploinsufficiency. Eur J Hum Genet. 25:94–99. 2016. View Article : Google Scholar : PubMed/NCBI
14	Pesch M, König S and Aumailley M: Targeted disruption of the Lama3 gene in adult mice is sufficient to induce skin inflammation and fibrosis. J Invest Dermatol. 137:332–340. 2017. View Article : Google Scholar : PubMed/NCBI
15	Arora E, Masab M, Jindal V, Riaz I, Gupta S and Varadi G: Role of poly adenosine diphosphate ribose polymerase inhibitors in advanced stage ovarian cancer. Cureus. 10:e26852018.PubMed/NCBI
16	Srinivas KG, Mirza AA, Swamy S, Amarendra S and Kodaganur G: Metachronous synchronous sternal and colonic metastasis with asymptomatic colo-colic fistula from carcinoma ovary rare presentation of ovarian cancer. Indian J Surg Oncol. 8:615–618. 2017. View Article : Google Scholar : PubMed/NCBI
17	Stone ML, Chiappinelli KB, Li H, Murphy LM, Travers ME, Topper MJ, Mathios D, Lim M, Shih IM, Wang TL, et al: Epigenetic therapy activates type I interferon signaling in murine ovarian cancer to reduce immunosuppression and tumor burden. Proc Natl Acad Sci USA. 114:E10981–E10990. 2017. View Article : Google Scholar : PubMed/NCBI
18	Stemmler S, Parwez Q, Petrasch-Parwez E, Epplen JT and Hoffjan S: Association of variation in the LAMA3 gene, encoding the alpha-chain of laminin 5, with atopic dermatitis in a German case-control cohort. BMC Dermatol. 14:172014. View Article : Google Scholar : PubMed/NCBI
19	Lincoln V, Cogan J, Hou Y, Hirsch M, Hao M, Alexeev V, De Luca M, De Rosa L, Bauer JW, Woodley DT, et al: Gentamicin induces LAMB3 nonsense mutation readthrough and restores functional laminin 332 in junctional epidermolysis bullosa. Proc Natl Acad Sci USA. 115:E6536–E6545. 2018. View Article : Google Scholar : PubMed/NCBI
20	Svoboda M, Hlobilová M, Marešová M, Sochorová M, Kováčik A, Vávrová K and Dolečková I: Comparison of suction blistering and tape stripping for analysis of epidermal genes, proteins and lipids. Arch Dermatol Res. 309:757–765. 2017. View Article : Google Scholar : PubMed/NCBI
21	Shukla DK, Gupta D, Aggarwal A and Kumar D: A case report of newly diagnosed epithelial ovarian carcinoma presenting with spontaneous tumor lysis syndrome and its successful management with rasburicase. Indian J Med Paediatr Oncol. 38:360–362. 2017. View Article : Google Scholar : PubMed/NCBI