Tanshinone IIA inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via p53‑cyclin B1/CDC2

Liu,Bin; Liu,Lixia; Zang,Aimin; Song,Zizheng; Yang,Hua; Wang,Zhiyu; Shang,Yanhong; Ma,Tao; Zhang,Yonggang

doi:10.3892/ol.2019.10658

Tanshinone IIA inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via p53‑cyclin B1/CDC2

Authors:
- Bin Liu
- Lixia Liu
- Aimin Zang
- Zizheng Song
- Hua Yang
- Zhiyu Wang
- Yanhong Shang
- Tao Ma
- Yonggang Zhang
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Affiliations: Department of Oncology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China, Department of Functions Branch, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China, Department of Surgery, Baoding No.1 Central Hospital, Baoding, Hebei 071000, P.R. China, Department of Surgery, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China
Published online on: July 24, 2019 https://doi.org/10.3892/ol.2019.10658
Pages: 3317-3322

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Abstract

Tanshinone IIA exhibits natural antioxidative and antineoplastic activity. However, to the best of our knowledge, the effects of tanshinone IIA on human nasopharyngeal carcinoma cells remains unknown. The present study aimed to investigate whether tanshinone IIA inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via p53‑cyclin B1/cell division cycle gene 2 (CDC2). Cell proliferation, cytotoxicity and apoptosis of 13‑9B cells were evaluated by an MTT assay, lactate dehydrogenase assay and flow cytometry, respectively. ELISA and western blot analysis were used to analyze caspase‑3 activity and poly (ADP‑ribose) polymerase (PARP), p53, cyclin B1 and CDC2 protein expression in 13‑9B cells. Treatment of 13‑9B cells with tanshinone IIA significantly suppressed cell proliferation and significantly induced cytotoxicity and apoptosis of 13‑9B cells. Furthermore, tanshinone IIA significantly increased caspase‑3 activity, and significantly increased the protein expression levels of PARP, p53, cyclin B1 and CDC2 in 13‑9B cells. In summary, the current results indicate that tanshinone IIA inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via PARP, p53, cyclin B1/CDC2 and caspase‑3‑mediated signaling.

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