miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway

Tang,Xiangqin; Ding,Yanjun; Wang,Xiaoqing; Wang,Xiuzhen; Zhao,Lin; Bi,Hongmei

doi:10.3892/ol.2019.10805

miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway

Authors:
- Xiangqin Tang
- Yanjun Ding
- Xiaoqing Wang
- Xiuzhen Wang
- Lin Zhao
- Hongmei Bi
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Affiliations: Department of Oncology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of General Surgery, People's Hospital of Chiping, Chiping, Shandong 252100, P.R. China, First Ward, Department of Neurology, The Affiliated Central Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Clinical Nutrition, People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Respiratory Medicine, People's Hospital of Rizhao, Rizhao, Shandong 276826, P.R. China, Department of Respiratory Medicine, The Third People's Hospital of Qingdao, Qingdao, Shandong 266041, P.R. China
Published online on: September 4, 2019 https://doi.org/10.3892/ol.2019.10805
Pages: 4621-4628
Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non-small cell lung cancer (NSCLC) is the most frequent cancer worldwide with a poor 5-year survival. miR-650 acts as an oncogene and regulates tumor progress in various cancers. Molecular mechanisms of miR-650 in NSCLC cell proliferation and invasion was studied. The mRNA levels of miR-650 and special genes were calculated using RT-qPCR. MTT and transwell assays were applied to measure the proliferative and invasive ability. Kaplan-Meier method was used to assess the survival of NSCLC patients. miR-650 was upregulated in NSCLC and upregulation of miR-650 was associated with a poor overall survival of NSCLC, while the results of ING4 demonstrated the opposite results. miR-650 promoted proliferation and invasion through Wnt-1/β‑catenin pathway by targeting inhibitor of growth 4 (ING4) in A549 cells. ING4 was a direct target gene of miR-650 and the expression of ING4 was mediated by exogenous altering the expression of miR-650. Remarkably, alterations of ING4 expression eliminated the functions of miR-650 on the proliferation and metastasis of NSCLC. miR-650 enhanced A549 cell proliferation and invasion through Wnt-1/β-catenin pathway by directly targeting the 3'-UTR of ING4 mRNA. The newly identified miR-650/ING4 axis provides a novel insight into the pathogenesis of NSCLC.

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