Characteristics of fecal gut microbiota in patients with colorectal cancer at different stages and different sites

Sheng,Qinsong; Du,Haixia; Cheng,Xiaofei; Cheng,Xiaobin; Tang,Yunning; Pan,Lelin; Wang,Qunmin; Lin,Jianjiang

doi:10.3892/ol.2019.10841

Characteristics of fecal gut microbiota in patients with colorectal cancer at different stages and different sites

Authors:
- Qinsong Sheng
- Haixia Du
- Xiaofei Cheng
- Xiaobin Cheng
- Yunning Tang
- Lelin Pan
- Qunmin Wang
- Jianjiang Lin
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Affiliations: Department of Colorectal and Anal Surgery, The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Institute of Cardio‑Cerebrovascular Diseases, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Colorectal and Anal Surgery, Changshan People' Hospital, Changshan, Zhejiang 324200, P.R. China
Published online on: September 10, 2019 https://doi.org/10.3892/ol.2019.10841
Pages: 4834-4844
Copyright: © Sheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Numerous studies have revealed that the gut microbiota serves an important role in the pathogenesis of colorectal cancer (CRC). The present study aimed to investigate the populations present in the gut microbiota in patients with CRC of different stages and at different sites. Fecal samples were obtained from 67 CRC patients and 30 healthy controls, which were analyzed by sequencing the V3‑V4 region of the 16S rRNA gene. Increased diversity of the fecal gut microbiota in patients with CRC was reported compared with the healthy controls. In the present study, at the genus level, the relative abundances of Prevotella, Collinsella and Peptostreptococcus in the gut microbiota of CRC patients were substantially increased compared with healthy controls, while the relative abundance of Escherichia‑Shigella was significantly lower. In addition, differences in the fecal gut microbiota were also compared between patients with stage I‑IV CRC and healthy controls. The results revealed that the abundances of the genera Peptostreptococcus, Collinsella and Ruminococcus were significantly increased in patients with CRC stage I compared with the healthy controls, while Alistipes was enriched in patients with stage III CRC compared with patients with stage IV. Furthermore, the present study reported that the genera Veillonella and Coprobacter were more abundant in the proximal segments than in the distal segments of the colon. In conclusion, despite the low number of samples employed in the present study, a signature of genera indicating dysbiosis of the gut microbiota of patients with stage I‑IV CRC patients was proposed, which may provide insight into the mechanisms underlying the progression of CRC. These findings are also valuable for developing novel fecal diagnostic methods and therapeutic strategies for the treatment of CRC.

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