Open Access

Targeting TM4SF5 with anti‑TM4SF5 monoclonal antibody suppresses the growth and motility of human pancreatic cancer cells

  • Authors:
    • Sangkyu Park
    • Dongbum Kim
    • Jeong‑A Park
    • Hyung‑Joo Kwon
    • Younghee Lee
  • View Affiliations

  • Published online on: November 21, 2019     https://doi.org/10.3892/ol.2019.11134
  • Pages: 641-650
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pancreatic cancer is one of the most lethal cancers. Transmembrane 4 superfamily member 5 protein (TM4SF5) is one of the candidate molecular targets used for the prevention and treatment of TM4SF5‑expressing cancers, including hepatocellular carcinoma, colon cancer and pancreatic cancer. Recently, a previous study reported the preventive effects of a peptide vaccine, which targeted TM4SF5, in a mouse pancreatic cancer model. The present study investigated the implication of TM4SF5 and the suppressive effect of anti‑human TM4SF5 monoclonal antibody (anti‑hTM4SF5 antibody) in human pancreatic cancer cell lines in vitro. Treatment with anti‑hTM4SF5 antibody reduced cell viability, modulated the expression of EMT markers Vimentin and E‑cadherin, and decreased cell motility in human pancreatic cancer cells that endogenously expressed TM4SF5. When TM4SF5 was exogenously overexpressed in the TM4SF5‑negative cell line, the cells indicated increased cell viability and motility compared with control cells, and the phenotype was reversed by anti‑hTM4SF5 antibody treatment. Therefore, the results of the current study demonstrated that the high expression of TM4SF5 is a tumorigenic factor in human pancreatic cells and anti‑hTM4SF5 antibody treatment exhibits a suppressive effect in TM4SF5‑expressing pancreatic cancer cells.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Park S, Kim D, Park JA, Kwon HJ and Lee Y: Targeting TM4SF5 with anti‑TM4SF5 monoclonal antibody suppresses the growth and motility of human pancreatic cancer cells. Oncol Lett 19: 641-650, 2020.
APA
Park, S., Kim, D., Park, J., Kwon, H., & Lee, Y. (2020). Targeting TM4SF5 with anti‑TM4SF5 monoclonal antibody suppresses the growth and motility of human pancreatic cancer cells. Oncology Letters, 19, 641-650. https://doi.org/10.3892/ol.2019.11134
MLA
Park, S., Kim, D., Park, J., Kwon, H., Lee, Y."Targeting TM4SF5 with anti‑TM4SF5 monoclonal antibody suppresses the growth and motility of human pancreatic cancer cells". Oncology Letters 19.1 (2020): 641-650.
Chicago
Park, S., Kim, D., Park, J., Kwon, H., Lee, Y."Targeting TM4SF5 with anti‑TM4SF5 monoclonal antibody suppresses the growth and motility of human pancreatic cancer cells". Oncology Letters 19, no. 1 (2020): 641-650. https://doi.org/10.3892/ol.2019.11134