CEP131 knockdown inhibits cell proliferation by inhibiting the ERK and AKT signaling pathways in non‑small cell lung cancer

Wang,Junying; Yang,Xiaoping; Han,Shixin; Zhang,Lizhi

doi:10.3892/ol.2020.11411

CEP131 knockdown inhibits cell proliferation by inhibiting the ERK and AKT signaling pathways in non‑small cell lung cancer

Authors:
- Junying Wang
- Xiaoping Yang
- Shixin Han
- Lizhi Zhang
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Affiliations: Department of Pathology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Anesthesiology, Dalian Obstetrics and Gynecology Hospital, Dalian, Liaoning 116033, P.R. China, Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Published online on: February 19, 2020 https://doi.org/10.3892/ol.2020.11411
Pages: 3145-3152
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Disrupted centrosome‑associated family protein expression can result in the detrimental duplication of centrosomes, causing genomic instability and subsequent carcinogenesis. Limited research has demonstrated that centrosomal protein 131 (CEP131) exhibits oncogenic activity in osteosarcoma, hepatocellular carcinoma and breast cancer. The present study demonstrated that there is an association between CEP131 expression and advanced Tumor‑Node‑Metastasis stage (P=0.016), and positive regional lymph node metastasis (P=0.023) in 91 cases of non‑small cell lung cancer. A549 and SPC‑A‑1 cells, with moderate expression levels of CEP131, were selected as representative cell lines. The results indicated that downregulation of CEP131 induced G1/S cell cycle arrest, inhibition of cyclins D1/E and cyclin‑dependent kinases 2/4/6, and induction of inhibitory p21/p27, all of which are regulated by ERK and AKT signaling, suggesting that CEP131 exhibits potential as a novel target in the treatment of lung cancer.

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