MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway

Zhang,Xinli; Xu,Jumei; Zhang,Hongchang; Sun,Jilan; Li,Na; Huang,Xianyu

doi:10.3892/ol.2020.11516

MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway

Authors:
- Xinli Zhang
- Jumei Xu
- Hongchang Zhang
- Jilan Sun
- Na Li
- Xianyu Huang
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Affiliations: Medical Records Management Office, Qingdao West Coast New Area Central Hospital, Qingdao, Shandong 266555, P.R. China, Department of Hepatobiliary Gastrointestinal Surgery, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Infectious Diseases, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Sterilization Supply Room, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Radiology, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Radiotherapy Center, Qingdao Central Hospital, Qingdao University, Qingdao, Shandong 266042, P.R. China
Published online on: April 6, 2020 https://doi.org/10.3892/ol.2020.11516
Pages: 3923-3930
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recently, a number of microRNAs (miRNAs) have been reported to play different roles in human cancers, including colorectal cancer (CRC). However, the specific role of miR‑758 has not been clarified in CRC. Therefore, the aim of the present study was to explore the role of miR‑758 in CRC. RT‑qPCR and western blot analysis were used to quantify the expression of miR‑758 and genes. The function of miR‑758 in CRC was investigated using Transwell, CCK‑8 and luciferase reporter assays. According to the results, the downregulation of miR‑758 expression was associated with aggressive behavior and poor prognosis in CRC patients. miR‑758 was shown to restrain the cell viability and metastasis in CRC. In addition, it was confirmed that miR‑758 directly targets PAX6 and inhibits CRC progression through targeting PAX6. The results also revealed that miR‑758 blocked EMT and PI3K/AKT pathway in CRC. In conclusion, miR‑758 acts as a tumor suppressor in CRC by downregulating PAX6.

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