Clinical significance of COL1A1 and COL1A2 expression levels in hypopharyngeal squamous cell carcinoma

Lin,Peiliang; Tian,Peng; Pang,Jiaqi; Lai,Lan; He,Gui; Song,Yang; Zheng,Yiqing

doi:10.3892/ol.2020.11594

Clinical significance of COL1A1 and COL1A2 expression levels in hypopharyngeal squamous cell carcinoma

Authors:
- Peiliang Lin
- Peng Tian
- Jiaqi Pang
- Lan Lai
- Gui He
- Yang Song
- Yiqing Zheng
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Affiliations: Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China
Published online on: May 7, 2020 https://doi.org/10.3892/ol.2020.11594
Pages: 803-809
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Alterations in collagen type I α1 (COL1A1) and collagen type I α 2 (COL1A2) expressionlevels have been reported to predict prognosis in various types of cancer. However, the effect of these biomarkers on hypopharyngeal squamous cell carcinoma (HPSCC) is yet to be fully elucidated. The present study aimed to explore the prognostic significance of COL1A1 and COL1A2 expression levels in HPSCC. The expression levels of COL1A1 and COL1A2 in 67 patients with HPSCC were examined using an immunohistochemical assay in a tissue microarray. The associations between COL1A1/COL1A2 expression levels and patient clinicopathological features were analyzed using ANOVA, Pearson's χ2 or Fisher's exact test. The Cox proportional hazard models and Kaplan‑Meier survival analysis with log‑rank tests were used to analyze the significance of COL1A1/COL1A2 as prognostic markers for patients with HPSCC. As a result, immunohistochemical staining revealed that COL1A1 was positively expressed in all cases, among which 40.3% were strong positive, while COL1A2 was positively expressed in 76.1% of the HPSCC cases with 6.0% of the samples exhibiting strong staining. Further analysis revealed no significant association between the expression levels of COL1A1/COL1A2 and other clinicopathological features. Cox regression analysis revealed that a high COL1A2 expression level predicted a high locoregional recurrence and a less favorable disease‑free survival rate (P=0.042 and 0.020, respectively). Overall, the present study indicated that COL1A2 expression levels may have value as a prognostic indicator in HPSCC.

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1	Pingree TF, Kim DR, Owen R and Larry D: Treatment of hypopharyngeal carcinoma: A 10-year review of 1,362 cases. Laryngoscope. 97:901–904. 1987. View Article : Google Scholar : PubMed/NCBI
2	Newman JR, Connolly TM, Illing EA, Kilgore ML, Locher JL and Carroll WR: Survival trends in hypopharyngeal cancer: A population-based review. Laryngoscope. 125:624–629. 2015. View Article : Google Scholar : PubMed/NCBI
3	Chu PY and Chang SY: Reconstruction of the hypopharynx after surgical treatment of squamous cell carcinoma. J Chin Med Assoc. 72:351–355. 2009. View Article : Google Scholar : PubMed/NCBI
4	Koga Y, Pelizzola M, Cheng E, Krauthammer M, Sznol M, Ariyan S, Narayan D, Molinaro AM, Halaban R and Weissman SM: Genome-wide screen of promoter methylation identifies novel markers in melanoma. Genome Res. 19:1462–1470. 2009. View Article : Google Scholar : PubMed/NCBI
5	Mori K, Enokida H, Kagara I, Kawakami K, Chiyomaru T, Tatarano S, Kawahara K, Nishiyama K, Seki N and Nakagawa M: CpG hypermethylation of collagen type I alpha 2 contributes to proliferation and migration activity of human bladder cancer. Int J Oncol. 34:1593–1602. 2009.PubMed/NCBI
6	Shin K, Lim A, Zhao C, Sahoo D, Pan Y, Spiekerkoetter E, Liao JC and Beachy PA: Hedgehog signaling restrains bladder cancer progression by eliciting stromal production of urothelial differentiation factors. Cancer Cell. 26:521–533. 2014. View Article : Google Scholar : PubMed/NCBI
7	Shin K, Lee J, Guo N, Kim J, Lim A, Qu L, Mysorekar IU and Beachy PA: Hedgehog/Wnt feedback supports regenerative proliferation of epithelial stem cells in bladder. Nature. 472:110–114. 2011. View Article : Google Scholar : PubMed/NCBI
8	Gao F, Li M, Xiang R, Zhou X, Zhu L and Zhai Y: Expression of CLDN6 in tissues of gastric cancer patients: Association with clinical pathology and prognosis. Oncol Lett. 17:4621–4625. 2019.PubMed/NCBI
9	Zhai JM, An YH, Wang W, Fan YG and Yao GL: IL-32 expression indicates unfavorable prognosis in patients with colon cancer. Oncol Lett. 17:4655–4660. 2019.PubMed/NCBI
10	Zhu YZ, Zhou K, Ruan LL, Sun F, Wang G and Li WF: Metadherin overexpression in perihilar cholangiocarcinoma is associated with lymph node metastasis and poor prognosis. Oncol Lett. 17:4514–4520. 2019.PubMed/NCBI
11	Zou X, Feng B, Dong T, Yan G, Tan B, Shen H, Huang A, Zhang X, Zhang M, Yang P, et al: Up-regulation of type I collagen during tumorigenesis of colorectal cancer revealed by quantitative proteomic analysis. J Proteomics. 94:473–485. 2013. View Article : Google Scholar : PubMed/NCBI
12	Liang Y, Diehn M, Bollen AW, Israel MA and Gupta N: Type I collagen is overexpressed in medulloblastoma as a component of tumor microenvironment. J Neurooncol. 86:133–141. 2008. View Article : Google Scholar : PubMed/NCBI
13	Misawa K, Kanazawa T, Misawa Y, Imai A, Endo S, Hakamada K and Mineta H: Hypermethylation of collagen α2 (I) gene (COL1A2) is an independent predictor of survival in head and neck cancer. Cancer Biomark. 10:135–144. 2011. View Article : Google Scholar : PubMed/NCBI
14	National Comprehensive Cancer Network (NCCN) clinical practice guidelines in oncology, . Head and Neck Cancer. Version 1. 2019.
15	Kononen J, Bubendorf L, Kallioniemi A, Bärlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G and Kallioniemi OP: Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med. 4:844–847. 1998. View Article : Google Scholar : PubMed/NCBI
16	Chen J, Li S, Xiao Y, Zou X, Zhang X, Zhu M, Cai M and Xie D: p53R2 as a novel prognostic biomarker in nasopharyngeal carcinoma. BMC Cancer. 17:8462017. View Article : Google Scholar : PubMed/NCBI
17	Carvalho AL, Nishimoto IN, Califano JA and Kowalski LP: Trends in incidence and prognosis for head and neck cancer in the United States: A site-specific analysis of the SEER database. Int J Cancer. 114:806–816. 2005. View Article : Google Scholar : PubMed/NCBI
18	Koo BS, Lim YC, Lee JS, Kim YH, Kim SH and Choi EC: Management of contralateral N0 neck in pyriform sinus carcinoma. Laryngoscope. 116:1268–1272. 2006. View Article : Google Scholar : PubMed/NCBI
19	Kuo P, Chen MM, Decker RH, Yarbrough WG and Judson BL: Hypopharyngeal cancer incidence, treatment, and survival: Temporal trends in the United States. Laryngoscope. 124:2064–2069. 2014. View Article : Google Scholar : PubMed/NCBI
20	Wang YL, Feng SH, Zhu J, Zhu J, Zhu GP, Li DS, Wang Y, Zhu YX, Sun GH and Ji QH: Impact of lymph node ratio on the survival of patients with hypopharyngeal squamous cell carcinoma: A population-based analysis. PLoS One. 8:e566132013. View Article : Google Scholar : PubMed/NCBI
21	Abrahão R, Anantharaman D, Gaborieau V, Abedi-Ardekani B, Lagiou P, Lagiou A, Ahrens W, Holcatova I, Betka J, Merletti F, et al: The influence of smoking, age and stage at diagnosis on the survival after larynx, hypopharynx and oral cavity cancers in Europe: The ARCAGE study. Int J Cancer. 143:32–44. 2018. View Article : Google Scholar : PubMed/NCBI
22	Yang J, Hsueh CY, Cao W and Zhou L: Pretreatment lymphocyte-to-monocyte ratio as an independent prognostic factor for hypopharyngeal squamous cell carcinoma. Acta Otolaryngol. 138:734–740. 2018. View Article : Google Scholar : PubMed/NCBI
23	Kuwahara T, Takahashi H, Sano D, Matsuoka M, Hyakusoku H, Hatano T, Hiiragi Y and Oridate N: Fibrinogen and neutrophil-to-lymphocyte ratio predicts survival in patients with advanced hypopharyngeal squamous cell carcinoma. Anticancer Res. 38:5321–5330. 2018. View Article : Google Scholar : PubMed/NCBI
24	Chen Y, Liu Y, Ni H, Ding C, Zhang X and Zhang Z: FoxM1 overexpression promotes cell proliferation and migration and inhibits apoptosis in hypopharyngeal squamous cell carcinoma resulting in poor clinical prognosis. Int J Oncol. 51:1045–1054. 2017. View Article : Google Scholar : PubMed/NCBI
25	Wu H, Chen J, Wang Q, Yin Y, Da P, Le H, Zhang Z and Qiu X: Abnormal expression of HAX-1 is associated with cellular proliferation and migration in human hypopharyngeal squamous cell carcinoma. Mol Med Rep. 16:4664–4670. 2017. View Article : Google Scholar : PubMed/NCBI
26	Lu P, Weaver VM and Werb Z: The extracellular matrix: A dynamic niche in cancer progression. J Cell Biol. 196:395–406. 2012. View Article : Google Scholar : PubMed/NCBI
27	Egeblad M, Rasch MG and Weaver VM: Dynamic interplay between the collagen scaffold and tumor evolution. Curr Opin Cell Biol. 22:697–706. 2010. View Article : Google Scholar : PubMed/NCBI
28	Bonazzi VF, Nancarrow DJ, Stark MS, Moser RJ, Boyle GM, Aoude LG, Schmidt C and Hayward NK: Cross-platform array screening identifies COL1A2, THBS1, TNFRSF10D and UCHL1 as genes frequently silenced by methylation in melanoma. PLoS One. 6:e261212011. View Article : Google Scholar : PubMed/NCBI
29	Zhuo C, Li X, Zhuang H, Tian S, Cui H, Jiang R, Liu C, Tao R and Lin X: Elevated THBS2, COL1A2, and SPP1 expression levels as predictors of gastric cancer prognosis. Cell Physiol Biochem. 40:1316–1324. 2016. View Article : Google Scholar : PubMed/NCBI
30	Rong L, Huang W, Tian S, Chi X, Zhao P and Liu F: COL1A2 is a novel biomarker to improve clinical prediction in human gastric cancer: Integrating bioinformatics and meta-analysis. Pathol Oncol Res. 24:129–134. 2018. View Article : Google Scholar : PubMed/NCBI
31	Lin J, Goldstein L, Nesbit A and Chen MY: Influence of hormone receptor status on spinal metastatic lesions in patients with breast cancer. World Neurosurg. 85:42–48. 2016. View Article : Google Scholar : PubMed/NCBI