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Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma

  • Authors:
    • Yijie Li
    • Xu Guo
    • Shanshan Guo
    • Yang Wang
    • Lin Chen
    • Yang Liu
    • Min Jia
    • Jiaze An
    • Kaishan Tao
    • Jinliang Xing
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, State Key Laboratory of Cancer Biology and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2820-2828
    |
    Published online on: July 8, 2020
       https://doi.org/10.3892/ol.2020.11831
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Abstract

Emerging evidence has revealed that mitochondrial DNA (mtDNA) is encapsulated in plasma extracellular vesicles (EVs). However, the characteristics of mtDNA in EVs from patients with cancer remain largely unexplored, which greatly limits its clinical application. Whole genome and capture‑based sequencing found that EV mtDNA covered the whole mitochondrial genome. The medium fragment size in EV mtDNA was significantly larger compared with that in cell‑free mtDNA [cfmtDNA; 159 vs. 109 base pairs (bp); P<0.001]. EV DNA appeared to have a higher mtDNA copy number compared with cfDNA. Of note, patients with hepatitis had >300‑bp fragments in EV mtDNA compared with patients with hepatocellular carcinoma (HCC) and healthy controls. EV mtDNA fragments >300 bp in length exhibited a significantly higher proportion of EV mtDNA fragment ends than those that were ≤300 bp in length in patients with hepatitis. The EV mtDNA copy number in patients with HCC and hepatitis were significantly lower compared with those in healthy controls. Furthermore, inconsistencies in the mtDNA heteroplasmic variant were observed among HCC tissues, plasma and EVs. In conclusion, EV mtDNA exhibited different characteristics among patients with HCC, hepatitis and healthy controls, indicating the potential value of EV mtDNA as a diagnostic biomarker that complements cfmtDNA.
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View References

1 

Tkach M and Thery C: Communication by extracellular vesicles: Where we are and where we need to go. Cell. 164:1226–1232. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Li X, Li C, Zhang L, Wu M, Cao K, Jiang F, Chen D, Li N and Li W: The significance of exosomes in the development and treatment of hepatocellular carcinoma. Mol Cancer. 19:12020. View Article : Google Scholar : PubMed/NCBI

3 

Xu R, Rai A, Chen M, Suwakulsiri W, Greening DW and Simpson RJ: Extracellular vesicles in cancer-implications for future improvements in cancer care. Nat Rev Clin Oncol. 15:617–638. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Cappello F, Logozzi M, Campanella C, Bavisotto CC, Marcilla A, Properzi F and Fais S: Exosome levels in human body fluids: A tumor marker by themselves? Eur J Pharm Sci. 96:93–98. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, LeBleu VS, Mittendorf EA, Weitz J, Rahbari N, et al: Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature. 523:177–182. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Li Y, Zheng Q, Bao C, Li S, Guo W, Zhao J, Chen D, Gu J, He X and Huang S: Circular RNA is enriched and stable in exosomes: A promising biomarker for cancer diagnosis. Cell Res. 25:981–984. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Duijvesz D, Luider T, Bangma CH and Jenster G: Exosomes as biomarker treasure chests for prostate cancer. Eur Urol. 59:823–831. 2011. View Article : Google Scholar : PubMed/NCBI

8 

van Niel G, D'Angelo G and Raposo G: Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 19:213–228. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Thakur BK, Zhang H, Becker A, Matei I, Huang Y, Costa-Silva B, Zheng Y, Hoshino A, Brazier H, Xiang J, et al: Double-stranded DNA in exosomes: A novel biomarker in cancer detection. Cell Res. 24:766–769. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Kahlert C, Melo SA, Protopopov A, Tang J, Seth S, Koch M, Zhang J, Weitz J, Chin L, Futreal A and Kalluri R: Identification of double-stranded genomic DNA spanning all chromosomes with mutated KRAS and p53 DNA in the serum exosomes of patients with pancreatic cancer. J Biol Chem. 289:3869–3875. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Wan Y, Liu B, Lei H, Zhang B, Wang Y, Huang H, Chen S, Feng Y, Zhu L, Gu Y, et al: Nanoscale extracellular vesicle-derived DNA is superior to circulating cell-free DNA for mutation detection in early-stage non-small-cell lung cancer. Ann Oncol. 29:2379–2383. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Allenson K, Castillo J, San Lucas FA, Scelo G, Kim DU, Bernard V, Davis G, Kumar T, Katz M, Overman MJ, et al: High prevalence of mutant KRAS in circulating exosome-derived DNA from early-stage pancreatic cancer patients. Ann Oncol. 28:741–747. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Jin Y, Chen K, Wang Z, Wang Y, Liu J, Lin L, Shao Y, Gao L, Yin H, Cui C, et al: DNA in serum extracellular vesicles is stable under different storage conditions. BMC Cancer. 16:7532016. View Article : Google Scholar : PubMed/NCBI

14 

Yu M: Somatic mitochondrial DNA mutations in human cancers. Adv Clin Chem. 57:99–138. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Yu M: Generation, function and diagnostic value of mitochondrial DNA copy number alterations in human cancers. Life Sci. 89:65–71. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Reznik E, Miller ML, Şenbabaoğlu Y, Riaz N, Sarungbam J, Tickoo SK, Al-Ahmadie HA, Lee W, Seshan VE, Hakimi AA and Sander C: Mitochondrial DNA copy number variation across human cancers. ELife. 5:e107692016. View Article : Google Scholar : PubMed/NCBI

17 

Zhao SY, Yang YF, Liu J, Liu HQ, Ge NJ, Yang HS, Zhang HX and Xing JL: Association of mitochondrial DNA content in peripheral blood leukocyte with hepatitis B virus-related hepatocellular carcinoma in a Chinese Han population. Cancer Sci. 102:1553–1558. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Yin C, Li DY, Guo X, Cao HY, Chen YB, Zhou F, Ge NJ, Liu Y, Guo SS, Zhao Z, et al: NGS-based profiling reveals a critical contributing role of somatic D-loop mtDNA mutations in HBV-related hepatocarcinogenesis. Ann Oncol. 30:953–962. 2019. View Article : Google Scholar : PubMed/NCBI

19 

Mair R, Mouliere F, Smith CG, Chandrananda D, Gale D, Marass F, Tsui DWY, Massie CE, Wright AJ, Watts C, et al: Measurement of plasma cell-free mitochondrial tumor DNA improves detection of glioblastoma in patient-derived orthotopic xenograft models. Cancer Res. 79:220–230. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Guescini M, Genedani S, Stocchi V and Agnati LF: Astrocytes and glioblastoma cells release exosomes carrying mtDNA. J Neural Transm (Vienna). 117:1–4. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Sansone P, Savini C, Kurelac I, Chang Q, Amato LB, Strillacci A, Stepanova A, Iommarini L, Mastroleo C, Daly L, et al: Packaging and transfer of mitochondrial DNA via exosomes regulate escape from dormancy in hormonal therapy-resistant breast cancer. Proc Natl Acad Sci U S A. 114:E9066–E9075. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Satala CB, Jung I, Kobori L, Kovacs Z, Fodor D, Szodorai R and Gurzu S: Benefits of the 8th American joint committee on cancer system for hepatocellular carcinoma staging. J Gastrointest Cancer. 2020.(Online ahead of print). View Article : Google Scholar : PubMed/NCBI

23 

Zhu X, Shen H, Yin X, Yang M, Wei H, Chen Q, Feng F, Liu Y, Xu W and Li Y: Macrophages derived exosomes deliver miR-223 to epithelial ovarian cancer cells to elicit a chemoresistant phenotype. J Exp Clin Cancer Res. 38:812019. View Article : Google Scholar : PubMed/NCBI

24 

Chen S, Zhou Y, Chen Y and Gu J: Fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 34:i884–i890. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G and Durbin R; 1000 Genome Project Data Processing Subgroup, : The sequence alignment/map format and SAMtools. Bioinformatics. 25:2078–2079. 2009. View Article : Google Scholar : PubMed/NCBI

26 

McKenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, Daly M and DePristo MA: The genome analysis toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20:1297–1303. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Maas SLN, Breakefield XO and Weaver AM: Extracellular vesicles: Unique intercellular delivery vehicles. Trends Cell Biol. 27:172–188. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Jahr S, Hentze H, Englisch S, Hardt D, Fackelmayer FO, Hesch RD and Knippers R: DNA fragments in the blood plasma of cancer patients: Quantitations and evidence for their origin from apoptotic and necrotic cells. Cancer Res. 61:1659–1665. 2001.PubMed/NCBI

29 

Mouliere F, Chandrananda D, Piskorz AM, Moore EK, Morris J, Ahlborn LB, Mair R, Goranova T, Marass F, Heider K, et al: Enhanced detection of circulating tumor DNA by fragment size analysis. Sci Transl Med. 10:eaat49212018. View Article : Google Scholar : PubMed/NCBI

30 

Underhill HR, Kitzman JO, Hellwig S, Welker NC, Daza R, Baker DN, Gligorich KM, Rostomily RC, Bronner MP and Shendure J: Fragment length of circulating tumor DNA. PLoS Genet. 12:e10061622016. View Article : Google Scholar : PubMed/NCBI

31 

Patrushev M, Kasymov V, Patrusheva V, Ushakova T, Gogvadze V and Gaziev A: Mitochondrial permeability transition triggers the release of mtDNA fragments. Cell Mol Life Sci. 61:3100–3103. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Qu C, Zhang S, Li Y, Wang Y, Peppelenbosch MP and Pan Q: Mitochondria in the biology, pathogenesis, and treatment of hepatitis virus infections. Rev Med Virol. 29:e20752019. View Article : Google Scholar : PubMed/NCBI

33 

McArthur K, Whitehead LW, Heddleston JM, Li L, Padman BS, Oorschot V, Geoghegan ND, Chappaz S, Davidson S, Chin HS, et al: BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis. Science. 359:eaao60472018. View Article : Google Scholar : PubMed/NCBI

34 

Zhang X, Wu X, Hu Q, Wu J, Wang G, Hong Z and Ren J; Lab for Trauma and Surgical Infections, : Mitochondrial DNA in liver inflammation and oxidative stress. Life Sci. 236:1164642019. View Article : Google Scholar : PubMed/NCBI

35 

Hsu CC, Lee HC and Wei YH: Mitochondrial DNA alterations and mitochondrial dysfunction in the progression of hepatocellular carcinoma. World J Gastroenterol. 19:8880–8886. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Lee HC, Li SH, Lin JC, Wu CC, Yeh DC and Wei YH: Somatic mutations in the D-loop and decrease in the copy number of mitochondrial DNA in human hepatocellular carcinoma. Mutat Res. 547:71–78. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Baysa A, Fedorov A, Kondratov K, Ruusalepp A, Minasian S, Galagudza M, Popov M, Kurapeev D, Yakovlev A, Valen G, et al: Release of mitochondrial and nuclear DNA during on-pump heart surgery: Kinetics and relation to extracellular vesicles. J Cardiovasc Transl Res. 12:184–192. 2019. View Article : Google Scholar : PubMed/NCBI

38 

Bernard V, Kim DU, San Lucas FA, Castillo J, Allenson K, Mulu FC, Stephens BM, Huang J, Semaan A, Guerrero PA, et al: Circulating Nucleic Acids Are Associated With Outcomes of Patients With Pancreatic Cancer. Gastroenterology. 156:108–118. 2019. View Article : Google Scholar : PubMed/NCBI

39 

Weerts MJA, Timmermans EC, van de Stolpe A, Vossen RHAM, Anvar SY, Foekens JA, Sleijfer S and Martens JWM: Tumor-specific mitochondrial DNA variants are rarely detected in cell-free DNA. Neoplasia. 20:687–696. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Guo X, Guo S, Wang Y, Chen L, Liu Y, Jia M, An J, Tao K, Xing J, Xing J, et al: Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma. Oncol Lett 20: 2820-2828, 2020.
APA
Li, Y., Guo, X., Guo, S., Wang, Y., Chen, L., Liu, Y. ... Xing, J. (2020). Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma. Oncology Letters, 20, 2820-2828. https://doi.org/10.3892/ol.2020.11831
MLA
Li, Y., Guo, X., Guo, S., Wang, Y., Chen, L., Liu, Y., Jia, M., An, J., Tao, K., Xing, J."Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma". Oncology Letters 20.3 (2020): 2820-2828.
Chicago
Li, Y., Guo, X., Guo, S., Wang, Y., Chen, L., Liu, Y., Jia, M., An, J., Tao, K., Xing, J."Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma". Oncology Letters 20, no. 3 (2020): 2820-2828. https://doi.org/10.3892/ol.2020.11831
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Guo X, Guo S, Wang Y, Chen L, Liu Y, Jia M, An J, Tao K, Xing J, Xing J, et al: Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma. Oncol Lett 20: 2820-2828, 2020.
APA
Li, Y., Guo, X., Guo, S., Wang, Y., Chen, L., Liu, Y. ... Xing, J. (2020). Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma. Oncology Letters, 20, 2820-2828. https://doi.org/10.3892/ol.2020.11831
MLA
Li, Y., Guo, X., Guo, S., Wang, Y., Chen, L., Liu, Y., Jia, M., An, J., Tao, K., Xing, J."Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma". Oncology Letters 20.3 (2020): 2820-2828.
Chicago
Li, Y., Guo, X., Guo, S., Wang, Y., Chen, L., Liu, Y., Jia, M., An, J., Tao, K., Xing, J."Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma". Oncology Letters 20, no. 3 (2020): 2820-2828. https://doi.org/10.3892/ol.2020.11831
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