Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia&nbsp;yezoensis</em> Sookwawon&nbsp;104 with <em>in&nbsp;vitro</em> antiproliferative activity

He,Dan; Yan,Liping; Ma,Xiaojing; Cheng,Yang; Wu,Siya; Zuo,Jihui; Park,Eun-Jeong; Liu,Jian; Wu,Mingjiang; Choi,Jong-Il; Tong,Haibin

doi:10.3892/ol.2020.11952

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain Pyropia yezoensis Sookwawon 104 with in vitro antiproliferative activity

Authors:
- Dan He
- Liping Yan
- Xiaojing Ma
- Yang Cheng
- Siya Wu
- Jihui Zuo
- Eun-Jeong Park
- Jian Liu
- Mingjiang Wu
- Jong-Il Choi
- Haibin Tong
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Affiliations: Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang 325035, P.R. China, National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, P.R. China, Seaweed Research Center, National Institute of Fisheries Science, Haenam, South Jeolla 59002, Republic of Korea, Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea
Published online on: August 6, 2020 https://doi.org/10.3892/ol.2020.11952
Article Number: 91

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Abstract

Pyropia yezoensis Sookwawon 104 is a newly cultivated strain of red marine algae. The present study aimed to investigate the in vitro antiproliferative activity of sulfated polysaccharide extracted from P. yezoensis Sookwawon 104 (PYSP), as well as that of its low molecular weight (Mw) derivatives. PYSP is a heterogeneous sulfated polysaccharide mainly composed of galactose, glucose and fucose. PYSP was degraded by gamma‑irradiation at doses of 20 and 100 kGy to produce two derivatives, named as PYSP‑20 and PYSP‑100, respectively. Comparison of PYSP, PYSP‑20 and PYSP‑100 revealed clear differences in their molecular weight (Mw) distributions, and distinct in vitro antiproliferative activities against Hep3B, MDA‑MB‑231 and HeLa cancer cell lines. PYSP‑20 and PYSP‑100 exhibited stronger antiproliferative effects than PYSP, suggesting that the reduction in Mw may have increased the in vitro antiproliferative activity. Furthermore, the mRNA expression levels of the antitumor gene P53 and cell cycle‑associated genes P21, Cyclin B1 and cyclin dependent kinase 1 (Cdk1) were further analyzed by reverse transcription‑quantitative PCR in PYSP‑20 and PYSP‑100‑treated cancer cells. PYSP and its derivatives were shown to inhibit the proliferation of tumor cells by regulating the expression of P53, P21, Cyclin B1 and Cdk1. In conclusion, low‑Mw polysaccharide derivatives prepared from P. yezoensis Sookwawon 104 by gamma‑irradiation exhibit significant inhibition effects on cancer cell proliferation in vitro and may be a novel source of potential anticancer therapeutic agents.

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