Open Access

TLR9 induces colitis‑associated colorectal carcinogenesis by regulating NF‑κB expression levels

  • Authors:
    • Qingtian Luo
    • Ling Zeng
    • Chaotao Tang
    • Zhendong Zhang
    • Youxiang Chen
    • Chunyan Zeng
  • View Affiliations

  • Published online on: August 12, 2020     https://doi.org/10.3892/ol.2020.11971
  • Article Number: 110
  • Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic colorectal inflammation has been associated with colorectal cancer (CRC); however, its exact molecular mechanisms remain unclear. The present study aimed to investigate the effect of Toll‑like receptor 9 (TLR9) on the development of colitis‑associated CRC (CAC) through its regulation of the NF‑κB signaling pathway. By using a CAC mouse model and immunohistochemistry, the present study discovered that the protein expression levels of TLR9 were gradually upregulated during the development of CRC. In addition, the expression levels of TLR9 were revealed to be positively correlated with NF‑κB and Ki67 expression levels. In vitro, inhibiting TLR9 expression levels using chloroquine decreased the cell viability, proliferation and migration of the CRC cell line HT29, and further experiments indicated that this may occur through downregulating the expression levels of NF‑κB, proliferating cell nuclear antigen and Bcl‑xl. In conclusion, the findings of the present study suggested that TLR9 may serve an important role in the development of CAC by regulating NF‑κB signaling.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Luo Q, Zeng L, Tang C, Zhang Z, Chen Y and Zeng C: TLR9 induces colitis‑associated colorectal carcinogenesis by regulating NF‑κB expression levels. Oncol Lett 20: 110, 2020.
APA
Luo, Q., Zeng, L., Tang, C., Zhang, Z., Chen, Y., & Zeng, C. (2020). TLR9 induces colitis‑associated colorectal carcinogenesis by regulating NF‑κB expression levels. Oncology Letters, 20, 110. https://doi.org/10.3892/ol.2020.11971
MLA
Luo, Q., Zeng, L., Tang, C., Zhang, Z., Chen, Y., Zeng, C."TLR9 induces colitis‑associated colorectal carcinogenesis by regulating NF‑κB expression levels". Oncology Letters 20.4 (2020): 110.
Chicago
Luo, Q., Zeng, L., Tang, C., Zhang, Z., Chen, Y., Zeng, C."TLR9 induces colitis‑associated colorectal carcinogenesis by regulating NF‑κB expression levels". Oncology Letters 20, no. 4 (2020): 110. https://doi.org/10.3892/ol.2020.11971