PLAC8 inhibits oral squamous cell carcinogenesis and epithelial-mesenchymal transition via the Wnt/&beta;-catenin and PI3K/Akt/GSK3&beta; signaling pathways

Wu,Junlu; Wang,Xuetao; Shang,Anquan; Vella,Giovanna; Sun,Zujun; Ji,Ping; Yang,Dianyu; Wan,Aiming; Yao,Yiwen; Li,Dong

doi:10.3892/ol.2020.11989

PLAC8 inhibits oral squamous cell carcinogenesis and epithelial-mesenchymal transition via the Wnt/β-catenin and PI3K/Akt/GSK3β signaling pathways

Authors:
- Junlu Wu
- Xuetao Wang
- Anquan Shang
- Giovanna Vella
- Zujun Sun
- Ping Ji
- Dianyu Yang
- Aiming Wan
- Yiwen Yao
- Dong Li
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Affiliations: Department of Clinical Laboratory, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China, Department of Radiology, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China, Department of Internal Medicine V Pulmonology, Allergology and Respiratory Critical Care Medicine, Saarland University, Homburg D-66421, Germany
Published online on: August 19, 2020 https://doi.org/10.3892/ol.2020.11989
Article Number: 128
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Placenta-specific 8 (PLAC8) is closely associated with the proliferation, apoptosis and autophagy of several tumor cells. However, the expression and function of PLAC8 in oral squamous cell carcinoma (OSCC) remain unknown. Therefore, the present study investigated the function and mechanism of PLAC8 in OSCC. Reverse transcription-quantitative PCR and western blot analyses were performed to quantify the expression of PLAC8 in OSCC cell lines. The function of PLAC8 in OSCC was investigated via transfection, the Transwell and Cell Counting Kit-8 assays, immunofluorescence staining and western blotting. The results demonstrated that PLAC8 exspression was downregulated in OSCC cell lines. PLAC8 inhibited the cell proliferation in OSCC. In addition, PLAC8 restrained invasion and epithelial-mesenchymal transition of OSCC cells. Furthermore, β-catenin helped to repress PLAC8 expression by regulating the Wnt/β-catenin and PI3K/Akt/GSK3β signaling pathways in OSCC cells. Collectively, the results of the present study suggest that PLAC8 acts as a tumor suppressor in OSCC by downregulating β-catenin.

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