Open Access

A three serum miRNA panel as diagnostic biomarkers of radiotherapy‑related metastasis in non‑small cell lung cancer

  • Authors:
    • Jin Lv
    • Juan An
    • Yang-Dong Zhang
    • Zhao-Xia Li
    • Guang-Li Zhao
    • Jun Gao
    • Wen-Wei Hu
    • Huo-Ming Chen
    • Ai-Min Li
    • Qi-Sheng Jiang
  • View Affiliations

  • Published online on: September 14, 2020     https://doi.org/10.3892/ol.2020.12099
  • Article Number: 236
  • Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Serum microRNAs (miRNAs) have been implicated as noninvasive biomarkers for lung cancer diagnosis. However, there are no sensitive and specific biomarkers for the detection of radiotherapy‑related non‑small cell lung cancer (NSCLC) metastasis. The present study aimed to investigate the role of three serum miRNAs, namely miRNA (miR)‑130a, miR‑25 and miR‑191*, in diagnosing NSCLC, and their biological functions in radiation‑mediated development of metastatic properties in A549 cells. To determine this, serum samples were collected from 84 patients with NSCLC and 42 age‑ and sex‑matched healthy controls. Differential expression of serum miRNAs was analyzed by quantitative PCR. Significant associations between miRNA expression and overall survival of patients with NSCLC were identified using the Cox proportional regression model. A receiver operating characteristic curve was generated to evaluate diagnostic accuracy. The functions of miR‑130a, miR‑25 and miR‑191* in lung cancer cells were studied by transfecting A549 cells with miRNA mimics and inhibitors. The results of the present study demonstrated that the expression levels of miR‑130a, miR‑25 and miR‑191* in the serum of patients with NSCLC were increased compared with those in healthy controls, and these increases were associated with advanced age (≥60 years), radiotherapy, histological type (squamous carcinoma), low survival rate and low median survival time. Additionally, irradiation induced the upregulation of miR‑130a, miR‑25 and miR‑191* expression in A549 cells in vitro and in a xenograft mouse model. Irradiation also promoted the invasiveness of A549 cells in vitro and metastasis in vivo. In conclusion, miR‑130a, miR‑25 and miR‑191* may be potential biomarkers for the diagnosis of patients with NSCLC and may serve oncogenic roles in radiation‑mediated metastasis of NSCLC.
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November-2020
Volume 20 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Lv J, An J, Zhang Y, Li Z, Zhao G, Gao J, Hu W, Chen H, Li A, Jiang Q, Jiang Q, et al: A three serum miRNA panel as diagnostic biomarkers of radiotherapy‑related metastasis in non‑small cell lung cancer. Oncol Lett 20: 236, 2020.
APA
Lv, J., An, J., Zhang, Y., Li, Z., Zhao, G., Gao, J. ... Jiang, Q. (2020). A three serum miRNA panel as diagnostic biomarkers of radiotherapy‑related metastasis in non‑small cell lung cancer. Oncology Letters, 20, 236. https://doi.org/10.3892/ol.2020.12099
MLA
Lv, J., An, J., Zhang, Y., Li, Z., Zhao, G., Gao, J., Hu, W., Chen, H., Li, A., Jiang, Q."A three serum miRNA panel as diagnostic biomarkers of radiotherapy‑related metastasis in non‑small cell lung cancer". Oncology Letters 20.5 (2020): 236.
Chicago
Lv, J., An, J., Zhang, Y., Li, Z., Zhao, G., Gao, J., Hu, W., Chen, H., Li, A., Jiang, Q."A three serum miRNA panel as diagnostic biomarkers of radiotherapy‑related metastasis in non‑small cell lung cancer". Oncology Letters 20, no. 5 (2020): 236. https://doi.org/10.3892/ol.2020.12099