Open Access

Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma

  • Authors:
    • Hong-Tao Li
    • Bing Wei
    • Zhou-Quan Li
    • Xiao Wang
    • Wen-Xian Jia
    • Yan-Zhen Xu
    • Jia-Yi Liu
    • Meng-Nan Shao
    • Sui-Xia Chen
    • Nan-Fang Mo
    • Dong Zhao
    • Wen-Pu Zuo
    • Jian Qin
    • Ping Li
    • Qin-Le Zhang
    • Xiao-Li Yang
  • View Affiliations

  • Published online on: September 30, 2020     https://doi.org/10.3892/ol.2020.12171
  • Article Number: 308
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aberrant DNA replication is one of the driving forces behind oncogenesis. Furthermore, minichromosome maintenance complex component 3 (MCM3) serves an essential role in DNA replication. Therefore, in the present study, the diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma (HCC) were investigated. By utilizing The Cancer Genome Atlas (TCGA) database, global MCM3 mRNA levels were assessed in HCC and normal liver tissues. Its effects were further analyzed by reverse transcription‑quantitative PCR (RT‑qPCR), western blotting and immunohistochemistry in 78 paired HCC and adjacent tissues. Functional and pathway enrichment analyses were performed using the Search Tool for the Retrieval of Interacting Genes database. The expression levels of proteins that interact with MCM3 were also analyzed using the TCGA database and RT‑qPCR. Finally, algorithms combining receiver operating characteristic (ROC) curves were constructed using binary logistic regression using the TCGA results. Increased MCM3 mRNA expression with high α‑fetoprotein levels and advanced Edmondson‑Steiner grade were found to be characteristic of HCC. Survival analysis revealed that high MCM3 expression was associated with poor outcomes in patients with HCC. In addition, MCM3 protein expression was associated with increased tumor invasion in HCC tissues. MCM3 and its interacting proteins were found to be primarily involved in DNA replication, cell cycle and a number of binding processes. Algorithms combining ROCs of MCM3 and its interacting proteins were found to have improved HCC diagnosis ability compared with MCM3 and other individual diagnostic markers. In conclusion, MCM3 appears to be a promising diagnostic biomarker for HCC. Additionally, the present study provides a basis for the multi‑gene diagnosis of HCC using MCM3.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li H, Wei B, Li Z, Wang X, Jia W, Xu Y, Liu J, Shao M, Chen S, Mo N, Mo N, et al: Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma. Oncol Lett 20: 308, 2020.
APA
Li, H., Wei, B., Li, Z., Wang, X., Jia, W., Xu, Y. ... Yang, X. (2020). Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma. Oncology Letters, 20, 308. https://doi.org/10.3892/ol.2020.12171
MLA
Li, H., Wei, B., Li, Z., Wang, X., Jia, W., Xu, Y., Liu, J., Shao, M., Chen, S., Mo, N., Zhao, D., Zuo, W., Qin, J., Li, P., Zhang, Q., Yang, X."Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma". Oncology Letters 20.6 (2020): 308.
Chicago
Li, H., Wei, B., Li, Z., Wang, X., Jia, W., Xu, Y., Liu, J., Shao, M., Chen, S., Mo, N., Zhao, D., Zuo, W., Qin, J., Li, P., Zhang, Q., Yang, X."Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma". Oncology Letters 20, no. 6 (2020): 308. https://doi.org/10.3892/ol.2020.12171