Open Access

Genome‑wide analysis of microRNA to evaluate prognostic markers in isolated cancer glands and surrounding stroma in high‑grade serous ovarian carcinoma

  • Authors:
    • Chie Sato
    • Mitsumasa Osakabe
    • Takayuki Nagasawa
    • Hiromu  Suzuki
    • Hiroaki Itamochi
    • Tsukasa Baba
    • Tamotsu Sugai
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  • Published online on: October 8, 2020     https://doi.org/10.3892/ol.2020.12198
  • Article Number: 338
  • Copyright: © Sato et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The molecular mechanisms responsible for the progression of ovarian cancer remain incompletely understood. By targeting multiple cancer‑related genes, microRNAs (miRNAs) have been identified as key regulators of cancer development and progression. In addition, the microenvironment, which constitutes cancer glands and the surrounding stromal tissue at the invasive front, has an important role in cancer progression. Using array‑based analysis of 14 cases (cohort 1), the aim of the present study was to evaluate global miRNA expression in cancerous glands and surrounding stromal tissues (isolated using a crypt isolation method), in order to identify potential prognostic markers of high‑grade serous carcinoma (HGSC). Reverse transcription‑quantitative PCR was also used to verify the results in cohort 1 (14 cases) and in 16 additional HGSC cases (cohort 2; verification cohort). Firstly, miRNA expression levels were compared between HGSC and normal samples among both the isolated cancer gland and stromal tissue samples. Secondly, miRNA expression was compared between HGSC cases with recurrence and those without recurrence among the isolated cancer gland and stromal tissue samples. The results revealed six and seven miRNAs identified in both of the aforementioned comparisons in isolated cancer glands and surrounding stromal tissue, respectively. Furthermore, downregulation of miRNA‑214‑3p in isolated cancer glands and downregulation of miRNA‑320c in the corresponding stromal tissue were associated with a decrease in disease‑free survival (without recurrence) in cohort 2. These findings indicated that specific miRNAs expressed in cancer cells and surrounding stromal cells of HGSC may be potential biomarkers predicting patient prognosis.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Sato C, Osakabe M, Nagasawa T, Suzuki H, Itamochi H, Baba T and Sugai T: Genome‑wide analysis of microRNA to evaluate prognostic markers in isolated cancer glands and surrounding stroma in high‑grade serous ovarian carcinoma. Oncol Lett 20: 338, 2020.
APA
Sato, C., Osakabe, M., Nagasawa, T., Suzuki, H., Itamochi, H., Baba, T., & Sugai, T. (2020). Genome‑wide analysis of microRNA to evaluate prognostic markers in isolated cancer glands and surrounding stroma in high‑grade serous ovarian carcinoma. Oncology Letters, 20, 338. https://doi.org/10.3892/ol.2020.12198
MLA
Sato, C., Osakabe, M., Nagasawa, T., Suzuki, H., Itamochi, H., Baba, T., Sugai, T."Genome‑wide analysis of microRNA to evaluate prognostic markers in isolated cancer glands and surrounding stroma in high‑grade serous ovarian carcinoma". Oncology Letters 20.6 (2020): 338.
Chicago
Sato, C., Osakabe, M., Nagasawa, T., Suzuki, H., Itamochi, H., Baba, T., Sugai, T."Genome‑wide analysis of microRNA to evaluate prognostic markers in isolated cancer glands and surrounding stroma in high‑grade serous ovarian carcinoma". Oncology Letters 20, no. 6 (2020): 338. https://doi.org/10.3892/ol.2020.12198