Open Access

Thiostrepton and miR‑216b synergistically promote osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1

  • Authors:
    • Xiaobing Cai
    • Wenyu Xiao
    • Juexin Shen
    • Hui Lian
    • Yi Lu
    • Xianmiao Liu
    • Jisheng Gu
  • View Affiliations

  • Published online on: October 29, 2020     https://doi.org/10.3892/ol.2020.12254
  • Article Number: 391
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is a common primary bone cancer that there are currently no effective treatment strategies for. Forkhead box M1 (FoxM1) is key in the development of osteosarcoma, and microRNA (miR)‑216b serves an antitumor role by targeting FoxM1. Moreover, thiostrepton (TST), a natural thiazole antibiotic, induces antitumor effects and specifically targets FoxM1. Therefore, the present study investigated whether thiostrepton and miR‑216b synergistically inhibited osteosarcoma cells by targeting FoxM1. The MTT assay, reverse transcription‑quantitative PCR, a dual‑luciferase reporter assay and flow cytometry were performed. Compared with the human osteoblast cell line hFOB1.19, miR‑216b expression was significantly downregulated in the osteosarcoma cell lines U2OS, MG63 and Saos‑2. By contrast, FoxM1 expression was significantly upregulated in osteosarcoma cell lines compared with the hFOB1.19 cell line. The results indicated that miR‑216b targeted the 3'‑untranslated region of FoxM1. Moreover, the results suggested that miR‑216b cooperated with TST to decrease cell cytotoxicity and increase cell apoptosis. In addition, miR‑216b cooperated with TST to increase Bax expression and decrease Bcl‑2 expression. In conclusion, the combination of TST and miR‑216b synergistically promoted osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1. Therefore, the present study suggested that the combination of TST and miR‑216b may serve as a promising therapeutic strategy for osteosarcoma.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cai X, Xiao W, Shen J, Lian H, Lu Y, Liu X and Gu J: Thiostrepton and miR‑216b synergistically promote osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1. Oncol Lett 20: 391, 2020
APA
Cai, X., Xiao, W., Shen, J., Lian, H., Lu, Y., Liu, X., & Gu, J. (2020). Thiostrepton and miR‑216b synergistically promote osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1. Oncology Letters, 20, 391. https://doi.org/10.3892/ol.2020.12254
MLA
Cai, X., Xiao, W., Shen, J., Lian, H., Lu, Y., Liu, X., Gu, J."Thiostrepton and miR‑216b synergistically promote osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1". Oncology Letters 20.6 (2020): 391.
Chicago
Cai, X., Xiao, W., Shen, J., Lian, H., Lu, Y., Liu, X., Gu, J."Thiostrepton and miR‑216b synergistically promote osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1". Oncology Letters 20, no. 6 (2020): 391. https://doi.org/10.3892/ol.2020.12254