Upregulated hsa_circ_0000129 expression promotes proliferation and migration of breast cancer cells

Zhang,Zhenghua; Shi,Zhan; Zhang,Su; Lu,Qiong; Wei,Haimin; Wu,Xueyong; Han,Li

doi:10.3892/ol.2021.12500

Upregulated hsa_circ_0000129 expression promotes proliferation and migration of breast cancer cells

Authors:
- Zhenghua Zhang
- Zhan Shi
- Su Zhang
- Qiong Lu
- Haimin Wei
- Xueyong Wu
- Li Han
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Affiliations: Department of Oncology, Jing'an District Centre Hospital of Shanghai, Huashan Hospital, Fudan University, Jing'an Branch, Shanghai 200040, P.R. China, Department of Clinical Oncology, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Traditional Chinese Medicine Jing'an District Centre Hospital of Shanghai, Huashan Hospital, Fudan University, Jing'an Branch, Shanghai 200040, P.R. China, Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China
Published online on: January 28, 2021 https://doi.org/10.3892/ol.2021.12500
Article Number: 239
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) are considered potential biomarkers in the pathogenesis and detection of several types of cancer. The present study aimed to investigate the role of hsa_circ_0000129 in the pathogenesis and molecular mechanism underlying breast cancer. A total of 68 pairs of breast cancer and corresponding paracancerous tissue samples, three different breast cancer cell lines (MCF‑7, MDA‑MB‑231 and MDA‑MB‑468) and a normal human breast cell line (MCF‑10A) were used to investigate the expression of hsa_circ_0000129. The effect of hsa_circ_0000129 on cell proliferation, migration and colony formation was assessed in MCF‑7 and MDA‑MB‑468 cells, along with the expression of enhancer of zeste homolog 2 (EZH2). The results demonstrated that hsa_circ_0000129 expression was significantly higher in breast cancer tissues compared with normal tissues. In addition, high hsa_circ_0000129 expression was significantly associated with lymph node metastasis and a higher tumor‑node‑metastasis stage. Comparisons between the breast cancer cell lines (MCF‑7, MDA‑MB‑231 and MDA‑MB‑468) and MCF‑10A cells indicated similar results. MCF‑7 cells overexpressed with hsa_circ_0000129 significantly increased cell proliferation, migration and colony formation compared with the negative control group, the effects of which were reversed following hsa_circ_0000129 knockdown in MDA‑MB‑468 cells. Furthermore, EZH2 expression was positively associated with hsa_circ_0000129 expression. Taken together, the results of the present study suggest that hsa_circ_0000129 may represent a promising prognostic biomarker for breast cancer. In addition, the role of hsa_circ_0000129 in breast cancer cell lines indicates a mechanism for tumorigenesis, as well as a potent target for the treatment of malignant progression.

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