Long non‑coding RNA TMPO‑AS1 serves as a tumor promoter in pancreatic carcinoma by regulating miR‑383‑5p/SOX11

Xue,Feng; Song,Xin; Zhang,Shuai; Niu,Meiwei; Cui,Yu; Wang,Ye; Zhao,Ting

doi:10.3892/ol.2021.12517

Long non‑coding RNA TMPO‑AS1 serves as a tumor promoter in pancreatic carcinoma by regulating miR‑383‑5p/SOX11

Authors:
- Feng Xue
- Xin Song
- Shuai Zhang
- Meiwei Niu
- Yu Cui
- Ye Wang
- Ting Zhao
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Affiliations: Department of General Surgery, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, Shandong 266000, P.R. China, Department of Oncology, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, Shandong 266000, P.R. China
Published online on: February 4, 2021 https://doi.org/10.3892/ol.2021.12517
Article Number: 255
Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The dysregulation of lncRNA TMPO antisense RNA 1 (TMPO‑AS1) has been detected in various malignant tumors. However, the role of lncRNA TMPO‑AS1 remains unclear in pancreatic carcinoma. The present study aimed to elucidate the functional mechanism of TMPO‑AS1 in pancreatic carcinoma. In the present study, RT‑qPCR, western blotting, MTT, Transwell, luciferase reporter and xenograft assays were used to investigate the role of lncRNA TMPO‑AS1 in pancreatic carcinoma. Upregulation of lncRNA TMPO‑AS1 was revealed in pancreatic carcinoma tissues and cells. Furthermore, knockdown of TMPO‑AS1 restrained cell proliferation and motility in pancreatic carcinoma. In addition, microRNA (miR)‑383‑5p acted as a ‘sponge’ for lncRNA TMPO‑AS1. The expression levels of lncRNA TMPO‑AS1 and miR‑383‑5p were mutually inhibited in pancreatic carcinoma. Moreover, miR‑383‑5p was revealed to directly target SRY‑related high‑mobility group box 11 (SOX11). Notably, SOX11 could promote the occurrence of pancreatic carcinoma by interacting with the lncRNA TMPO‑AS1/miR‑383‑5p axis. In conclusion, upregulation of lncRNA TMPO‑AS1 promoted tumor growth, cell migration and invasion in pancreatic carcinoma by downregulating miR‑383‑5p and upregulating SOX11.

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