Open Access

PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma

  • Authors:
    • Yushen Su
    • Zhirui Zeng
    • Dongyun Rong
    • Yushi Yang
    • Bei Wu
    • Yu Cao
  • View Affiliations

  • Published online on: February 12, 2021     https://doi.org/10.3892/ol.2021.12550
  • Article Number: 289
  • Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The prognosis of patients with human papillomavirus (HPV)‑negative head and neck squamous cell carcinoma (HNSCC) is poorer than those with HPV‑positive HNSCC. The present study aimed to identify novel and specific biomarkers of HPV‑negative HNSCC using bioinformatics analysis and associated experiments. The gene expression profiles of HPV‑negative HNSCC tissues and corresponding clinical data were downloaded from The Cancer Genome Atlas database and used in a weighted gene co‑expression network analysis. Genes in clinically significant co‑expression modules were used to construct a protein‑protein interaction (PPI) network. The genes demonstrating a high degree score in the PPI network and a high correlation with tumor grade were considered hub genes. The diagnostic value of the hub genes associated with HPV‑negative and HPV‑positive HNSCC was analyzed using differential expression gene (DEG) analysis, immunohistochemical (IHC) staining and a receiver operating characteristic (ROC) curve analysis. Seven genes [Serrate RNA effector molecule (SRRT), checkpoint kinase 2 (CHEK2), small nuclear ribonucleoprotein polypeptide E (SNRPE), proteasome 26S subunit ATPase 2 (PSMC2), origin recognition complex subunit 5 (ORC5), S100 calcium binding protein A7 and keratinocyte differentiation associated protein (KRTDAP)] were demonstrated to be hub genes in clinically significant co‑expression modules. DEG, IHC and ROC curve analyses revealed that SRRT, CHEK2 and SNRPE were significantly upregulated in HPV‑negative and HPV‑positive HNSCC tissues compared with in adjacent tissues, and these genes demonstrated a high diagnostic value for distinguishing HNSCC tissues. However, PSMC2, ORC5 and KRTDAP were the only differentially expressed genes identified in HPV‑negative HNSCC tissues, and these genes demonstrated a high diagnostic value for HPV‑negative HNSCC. PSMC2, ORC5 and KRTDAP may therefore serve as novel and specific biomarkers for HPV‑negative HNSCC, potentially improving the diagnosis and treatment of patients with HPV‑negative HNSCC.
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April-2021
Volume 21 Issue 4

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Spandidos Publications style
Su Y, Zeng Z, Rong D, Yang Y, Wu B and Cao Y: PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma. Oncol Lett 21: 289, 2021.
APA
Su, Y., Zeng, Z., Rong, D., Yang, Y., Wu, B., & Cao, Y. (2021). PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma. Oncology Letters, 21, 289. https://doi.org/10.3892/ol.2021.12550
MLA
Su, Y., Zeng, Z., Rong, D., Yang, Y., Wu, B., Cao, Y."PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma". Oncology Letters 21.4 (2021): 289.
Chicago
Su, Y., Zeng, Z., Rong, D., Yang, Y., Wu, B., Cao, Y."PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma". Oncology Letters 21, no. 4 (2021): 289. https://doi.org/10.3892/ol.2021.12550