Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway

Chen,Deqin; Tang,Pengting; Wang,Yike; Wan,Fang; Long,Jingpei; Zhou,Jun; Zhuang,Ming; Chen,Xin

doi:10.3892/ol.2021.12634

Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway

Authors:
- Deqin Chen
- Pengting Tang
- Yike Wang
- Fang Wan
- Jingpei Long
- Jun Zhou
- Ming Zhuang
- Xin Chen
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Affiliations: Department of Surgery, The Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China, Department of Surgery, Ninghai Maternity and Child Care Hospital, Ningbo, Zhejiang 315600, P.R. China, Department of Breast Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China
Published online on: March 13, 2021 https://doi.org/10.3892/ol.2021.12634
Article Number: 373
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Extensive research has contributed to the current understanding of the critical roles played by long non‑coding RNAs in various types of cancer. The present study aimed to investigate the function and mechanism of the long non‑coding RNA, MIR4435‑2HG (also termed LINC00978), in breast cancer growth and metastasis. Using Gene Expression Profiling Interactive Analysis, an online web tool, it was revealed that MIR4435‑2HG was upregulated in breast cancer tissue, and its high expression was associated with poor prognosis based on The Cancer Genome Atlas database. MIR4435‑2HG knockdown increased cell apoptosis but decreased cell proliferation, migration and invasion. MIR4435‑2HG knockdown increased pro‑apoptotic protein expression but decreased anti‑apoptotic protein expression. In addition, MIR4435‑2HG knockdown leads to dysregulation of epithelial‑to‑mesenchymal transition‑associated genes. Furthermore, knockdown of MIR4435‑2HG results in inactivation of the Wnt/β‑catenin signaling pathway. The results of the present study demonstrate the tumor‑promoting role of MIR4435‑2HG in breast cancer progression.

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