Open Access

MicroRNA‑217 inhibits the proliferation and invasion, and promotes apoptosis of non‑small cell lung cancer cells by targeting sirtuin 1

  • Authors:
    • Guangshun Li
    • Shouping Zhong
  • View Affiliations

  • Published online on: March 17, 2021     https://doi.org/10.3892/ol.2021.12647
  • Article Number: 386
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Non‑small cell lung cancer (NSCLC) is a common malignancy worldwide. MicroRNA (miR)‑217 and sirtuin 1 (SIRT1) have been reported to play significant roles in different types of cancer, such as osteosarcoma and prostate cancer; however, the association between miR‑217 and SIRT1 in the cell proliferation, apoptosis and invasion of NSCLC remain unknown. Thus, the present study aimed to investigate the roles of miR‑217 and SIRT1 in NSCLC. The expression levels of miR‑217 and SIRT1 were detected via reverse transcription‑quantitative (RT‑q)PCR and western blot analyses. The effect of miR‑217 on A549 and H1299 cell proliferation, apoptosis and invasion was assessed via the Cell Counting Kit‑8, flow cytometry and Transwell assays, respectively. In addition, the association between SIRT1 and miR‑217 was predicted using the TargetScan database, and verified via the dual‑luciferase reporter assay, and RT‑qPCR and western blot analyses. The results demonstrated that miR‑217 expression was significantly downregulated, while SIRT1 expression was significantly upregulated in A549 and H1299 cells compared with the human bronchial epithelial cells. Furthermore, transfection with miR‑217 mimic significantly inhibited A549 and H1299 cell proliferation and invasion, and induced A549 and H1299 cell apoptosis. The results of the dual‑luciferase reporter assay and western blot analysis confirmed that SIRT1 is a target gene of miR‑217. In addition, miR‑217 inhibited the activation of AMP‑activated protein kinase (AMPK) and mTOR signaling. Taken together, the results of the present study suggest that miR‑217 inhibits A549 and H1299 cell proliferation and invasion, and induces A549 and H1299 cell apoptosis by targeting SIRT1 and inactivating the SIRT1‑mediated AMPK/mTOR signaling pathway. Thus, miR‑217 may be used as a potential therapeutic target for the treatment of patients with NSCLC.
View Figures
View References

Related Articles

Journal Cover

May-2021
Volume 21 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li G and Zhong S: MicroRNA‑217 inhibits the proliferation and invasion, and promotes apoptosis of non‑small cell lung cancer cells by targeting sirtuin 1. Oncol Lett 21: 386, 2021.
APA
Li, G., & Zhong, S. (2021). MicroRNA‑217 inhibits the proliferation and invasion, and promotes apoptosis of non‑small cell lung cancer cells by targeting sirtuin 1. Oncology Letters, 21, 386. https://doi.org/10.3892/ol.2021.12647
MLA
Li, G., Zhong, S."MicroRNA‑217 inhibits the proliferation and invasion, and promotes apoptosis of non‑small cell lung cancer cells by targeting sirtuin 1". Oncology Letters 21.5 (2021): 386.
Chicago
Li, G., Zhong, S."MicroRNA‑217 inhibits the proliferation and invasion, and promotes apoptosis of non‑small cell lung cancer cells by targeting sirtuin 1". Oncology Letters 21, no. 5 (2021): 386. https://doi.org/10.3892/ol.2021.12647