Open Access

Recombinant protein TRAIL-Mu3 enhances the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway

  • Authors:
    • Min Huang
    • Cheng Yi
    • Xian-Zhou Huang
    • Juan Yan
    • Li-Jia Wei
    • Wei-Ju Tang
    • Shou-Chun Chen
    • Ying Huang
  • View Affiliations

  • Published online on: April 1, 2021     https://doi.org/10.3892/ol.2021.12699
  • Article Number: 438
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pancreatic cancer is a highly malignant type of cancer and its treatment remains a major challenge. The novel recombinant protein TNF-related apoptosis-inducing ligand (TRAIL)-Mu3 has been shown to exert stronger tumor inhibitory effects in colon cancer in vitro and in vivo compared with TRAIL. The present study investigated the antitumor effects of TRAIL-Mu3 on pancreatic cancer cells, and the possible mechanisms were further examined. Compared with TRAIL, TRAIL-Mu3 exhibited significantly higher cytotoxic effects on pancreatic cancer cell lines. The inhibitory effect of TRAIL-Mu3 on the viability of PANC-1 cells was shown to be a caspase-dependent process. The affinity of TRAIL-Mu3 to PANC-1 cell membranes was significantly enhanced compared with TRAIL. In addition, TRAIL-Mu3 upregulated death receptor (DR) expression in PANC-1 cells and promoted the redistribution of DR5 in lipid rafts. Western blotting results demonstrated that TRAIL-Mu3 activated the caspase cascade in a faster and more efficient manner compared with TRAIL in PANC-1 cells. Therefore, TRAIL-Mu3 enhanced the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway. The present study indicated the potential of TRAIL-Mu3 for the treatment of pancreatic cancer.
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June-2021
Volume 21 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Huang M, Yi C, Huang X, Yan J, Wei L, Tang W, Chen S and Huang Y: Recombinant protein TRAIL-Mu3 enhances the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway. Oncol Lett 21: 438, 2021.
APA
Huang, M., Yi, C., Huang, X., Yan, J., Wei, L., Tang, W. ... Huang, Y. (2021). Recombinant protein TRAIL-Mu3 enhances the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway. Oncology Letters, 21, 438. https://doi.org/10.3892/ol.2021.12699
MLA
Huang, M., Yi, C., Huang, X., Yan, J., Wei, L., Tang, W., Chen, S., Huang, Y."Recombinant protein TRAIL-Mu3 enhances the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway". Oncology Letters 21.6 (2021): 438.
Chicago
Huang, M., Yi, C., Huang, X., Yan, J., Wei, L., Tang, W., Chen, S., Huang, Y."Recombinant protein TRAIL-Mu3 enhances the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway". Oncology Letters 21, no. 6 (2021): 438. https://doi.org/10.3892/ol.2021.12699