Open Access

Regulatory effect of microRNA‑223‑3p on breast cancer cell processes via the Hippo/Yap signaling pathway

  • Authors:
    • Tonghua Du
    • Dan Wang
    • Xiaoyu Wan
    • Jingwei Xu
    • Qi Xiao
    • Bin Liu
  • View Affiliations

  • Published online on: May 6, 2021     https://doi.org/10.3892/ol.2021.12777
  • Article Number: 516
  • Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

According to the 2018 global cancer statistics, the incidence and mortality rates of breast cancer are increasing gradually, which seriously threatens the health of women. MicroRNA‑223‑3p (miR‑223‑3p) can promote the proliferation and invasion of breast cancer cells. Hippo/Yes‑related protein (Yap) signaling pathway activation has been found in a variety of tumors. The present study aimed to investigate the potential mechanism of miR‑223‑3p in breast cancer. The Cell Counting Kit‑8 assay was used to detect cell viability and flow cytometry was used to detect apoptosis. The abilities of cell migration and invasion were detected using scratch and Transwell assays, as well as reverse transcription‑quantitative PCR and western blotting to detect gene and protein expression, respectively. The current results demonstrated that miR‑223‑3p transcription levels were increased in breast cancer cells, and inhibition of miR‑223‑3p gene expression decreased cell proliferation, migration and invasion. Additionally, inhibition of miR‑223‑3p expression inhibited epithelial‑mesenchymal transition (EMT) in breast cancer cells. miR‑223‑3p promoted cell proliferation, migration, invasion and EMT, and the western blotting results demonstrated that miR‑223‑3p inhibition increased the phosphorylation of Yap1 and the protein expression levels of large tumor suppressor kinase 1. In conclusion, results from the present results suggested that miR‑223‑3p may promote cell proliferation, migration, invasion and EMT through the Hippo/Yap signaling pathway. Therefore, miR‑223‑3p may be a potential biomarker for breast cancer.
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July-2021
Volume 22 Issue 1

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Copy and paste a formatted citation
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Spandidos Publications style
Du T, Wang D, Wan X, Xu J, Xiao Q and Liu B: Regulatory effect of microRNA‑223‑3p on breast cancer cell processes via the Hippo/Yap signaling pathway. Oncol Lett 22: 516, 2021
APA
Du, T., Wang, D., Wan, X., Xu, J., Xiao, Q., & Liu, B. (2021). Regulatory effect of microRNA‑223‑3p on breast cancer cell processes via the Hippo/Yap signaling pathway. Oncology Letters, 22, 516. https://doi.org/10.3892/ol.2021.12777
MLA
Du, T., Wang, D., Wan, X., Xu, J., Xiao, Q., Liu, B."Regulatory effect of microRNA‑223‑3p on breast cancer cell processes via the Hippo/Yap signaling pathway". Oncology Letters 22.1 (2021): 516.
Chicago
Du, T., Wang, D., Wan, X., Xu, J., Xiao, Q., Liu, B."Regulatory effect of microRNA‑223‑3p on breast cancer cell processes via the Hippo/Yap signaling pathway". Oncology Letters 22, no. 1 (2021): 516. https://doi.org/10.3892/ol.2021.12777