Open Access

MicroRNA profiles in five pairs of early gastric cancer tissues and adjacent non‑cancerous tissues

  • Authors:
    • Yuan Liang
    • Yongxun Zhao
    • Longquan Li
    • Hui Wei
    • Taobi Huang
    • Huiyun Zhang
    • Xia Chen
    • Heng Yun
    • Weiming Sun
    • Yuping Wang
  • View Affiliations

  • Published online on: June 7, 2021     https://doi.org/10.3892/ol.2021.12856
  • Article Number: 595
  • Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Approximately half of the world's gastric cancer cases and deaths occur in China. In addition, the incidence and mortality rates of gastric cancer in Gansu province in China are much higher than the average nationwide levels. The present study investigated microRNA (miRNA/miR) profiles in early gastric cancer (EGC) without specific symptoms. miRNA expression levels in five pairs of EGC tissues and adjacent non‑cancerous mucosa tissues of patients from Gansu province in China were analyzed using a miRNA microarray. A total of 47 differentially expressed miRNAs (DEMs) were identified. Subsequently, mRNA expression profiles of three pairs of cancer tissues and adjacent non‑cancerous tissues from 3 Asian patients with stage I or stage II gastric cancer (stage I/II; American Joint Committee on Cancer classification, Eighth Edition) were obtained from The Cancer Genome Atlas database, and differentially expressed genes (DEGs) were identified. The target genes of DEMs were filtered from the DEGs using the miRDB database and a miRNA‑gene network was constructed. The functions of DEMs were evaluated using the tool for annotations of human miRNAs database, and via Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis and Gene Set Enrichment Analysis of the target genes. Finally, survival analyses of DEMs, which were in the miRNA‑gene network, was performed. The results suggested that a number of miRNAs, including hsa‑let‑7a‑5p, hsa‑miR‑27a‑3p, hsa‑miR‑126‑5p and hsa‑miR‑424‑5p, may serve critical roles in EGC. The present study could provide a basis for the identification of EGC screening biomarkers. Furthermore, the present study may provide a basis for the exploration of the cause of the high incidence of gastric cancer in Gansu province from the perspective of miRNAs.
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August-2021
Volume 22 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Liang Y, Zhao Y, Li L, Wei H, Huang T, Zhang H, Chen X, Yun H, Sun W, Wang Y, Wang Y, et al: MicroRNA profiles in five pairs of early gastric cancer tissues and adjacent non‑cancerous tissues. Oncol Lett 22: 595, 2021.
APA
Liang, Y., Zhao, Y., Li, L., Wei, H., Huang, T., Zhang, H. ... Wang, Y. (2021). MicroRNA profiles in five pairs of early gastric cancer tissues and adjacent non‑cancerous tissues. Oncology Letters, 22, 595. https://doi.org/10.3892/ol.2021.12856
MLA
Liang, Y., Zhao, Y., Li, L., Wei, H., Huang, T., Zhang, H., Chen, X., Yun, H., Sun, W., Wang, Y."MicroRNA profiles in five pairs of early gastric cancer tissues and adjacent non‑cancerous tissues". Oncology Letters 22.2 (2021): 595.
Chicago
Liang, Y., Zhao, Y., Li, L., Wei, H., Huang, T., Zhang, H., Chen, X., Yun, H., Sun, W., Wang, Y."MicroRNA profiles in five pairs of early gastric cancer tissues and adjacent non‑cancerous tissues". Oncology Letters 22, no. 2 (2021): 595. https://doi.org/10.3892/ol.2021.12856