Open Access

Restoration of the ATG5‑dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs

  • Authors:
    • Ke Peng
    • Aiqin Sun
    • Jun Zhu
    • Jinyi Gao
    • Yanlin Li
    • Genbao Shao
    • Wannian Yang
    • Qiong Lin
  • View Affiliations

  • Published online on: July 6, 2021     https://doi.org/10.3892/ol.2021.12899
  • Article Number: 638
  • Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Autophagy serves an important role in cancer cell survival and drug resistance. In the present study, the prostate cancer DU145 cell line was used, which lacks autophagy related 5 (ATG5) expression and is defective in induction of ATG5‑dependent autophagy. The aim of the study was to examine the effects of the restoration of autophagy on cell proliferation and migration, and to assess the cytotoxicity caused by chemotherapeutic drugs, using microscopic, wound‑healing, western blot and apoptotic assays. The restoration of the autophagic activity in DU145 cells by the overexpression of ATG5 enhanced the cell proliferation and migration rates. Notably, restoration of the ATG5‑dependent autophagy in DU145 cells significantly increased the cytotoxic effects of the chemotherapeutic drugs, docetaxel and valproic acid, and the endoplasmic reticulum stress inducers, brefeldin A, tunicamycin and thapsigargin. The present study provides a novel perspective on the role of ATG5‑dependent autophagy in drug resistance and chemotherapy.
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September-2021
Volume 22 Issue 3

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Online ISSN:1792-1082

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Spandidos Publications style
Peng K, Sun A, Zhu J, Gao J, Li Y, Shao G, Yang W and Lin Q: Restoration of the ATG5‑dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs. Oncol Lett 22: 638, 2021.
APA
Peng, K., Sun, A., Zhu, J., Gao, J., Li, Y., Shao, G. ... Lin, Q. (2021). Restoration of the ATG5‑dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs. Oncology Letters, 22, 638. https://doi.org/10.3892/ol.2021.12899
MLA
Peng, K., Sun, A., Zhu, J., Gao, J., Li, Y., Shao, G., Yang, W., Lin, Q."Restoration of the ATG5‑dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs". Oncology Letters 22.3 (2021): 638.
Chicago
Peng, K., Sun, A., Zhu, J., Gao, J., Li, Y., Shao, G., Yang, W., Lin, Q."Restoration of the ATG5‑dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs". Oncology Letters 22, no. 3 (2021): 638. https://doi.org/10.3892/ol.2021.12899