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MicroRNA‑27b‑3p inhibits the proliferation and invasion of cutaneous squamous cell carcinoma by targeting EGFR and MMP‑13

  • Authors:
    • Daning Liang
    • Zhenning Zhang

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  • Published online on: August 10, 2021     https://doi.org/10.3892/ol.2021.12990
  • Article Number: 729
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Abstract

Cutaneous squamous cell carcinoma is a common malignant tumor. The aim of the present study was to examine the biological function of microRNA (miR)‑27b‑3p in cutaneous squamous cell carcinoma (CSCC) and its underlying mechanism. The relative expression levels of miR‑27b‑3p were determined in A‑431, Colo‑16 and NHEK/SVTERT3‑5 cell lines. The regulatory effects of miR‑27b‑3p on the proliferation of CSCC cells were evaluated using MTT and colony formation assays. Transwell assays were conducted to examine the role of miR‑27b‑5p in the migratory and invasive abilities of CSCC cells. The levels of EGFR, MMP‑13, Akt, phosphorylated (p)‑Akt, cyclin D1, N‑cadherin (CAD) and E‑CAD were detected in CSCC cells using reverse transcription‑quantitative PCR and western blot analysis. Binding between miR‑27b‑3p and the 3'‑untranslated region (UTR) of EGFR or MMP‑13 was assessed using a dual‑luciferase reporter assay. miR‑27b‑3p was significantly downregulated in CSCC cell lines, compared with the skin keratinocyte cell line. Transfection with a miR‑27b‑3p mimic significantly reduced the proliferative, migratory and invasive abilities of CSCC cells in vitro. Moreover, miR‑27b‑3p mimic transfection downregulated the mRNA and protein levels of EGFR, MMP‑13, cyclin D1, p‑Akt and N‑CAD, whilst upregulating E‑CAD levels in CSCC cells. miR‑27b‑3p was found to target the EGFR and MMP‑13 3'‑UTRs, thus downregulating the expression of these molecules. The inhibition of CSCC proliferation by miR‑27b‑3p was effectively reversed by EGFR overexpression. Moreover, the inhibitory effect of miR‑27b‑3p on the migratory and invasive abilities of CSCC cells was abolished by MMP‑13 overexpression. In conclusion, miR‑27b‑3p inhibits the proliferation, migration and invasion of CSCC cells by downregulating the expression of EGFR and MMP‑13 and may represent a potential diagnostic marker and therapeutic option for CSCC.
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October-2021
Volume 22 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • Impact Factor: 2.5
  • Ranked Oncology
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  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
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Spandidos Publications style
Liang D and Zhang Z: MicroRNA‑27b‑3p inhibits the proliferation and invasion of cutaneous squamous cell carcinoma by targeting EGFR and MMP‑13. Oncol Lett 22: 729, 2021.
APA
Liang, D., & Zhang, Z. (2021). MicroRNA‑27b‑3p inhibits the proliferation and invasion of cutaneous squamous cell carcinoma by targeting EGFR and MMP‑13. Oncology Letters, 22, 729. https://doi.org/10.3892/ol.2021.12990
MLA
Liang, D., Zhang, Z."MicroRNA‑27b‑3p inhibits the proliferation and invasion of cutaneous squamous cell carcinoma by targeting EGFR and MMP‑13". Oncology Letters 22.4 (2021): 729.
Chicago
Liang, D., Zhang, Z."MicroRNA‑27b‑3p inhibits the proliferation and invasion of cutaneous squamous cell carcinoma by targeting EGFR and MMP‑13". Oncology Letters 22, no. 4 (2021): 729. https://doi.org/10.3892/ol.2021.12990