Open Access

CTBP1 strengthens the cisplatin resistance of gastric cancer cells by upregulating RAD51 expression

  • Authors:
    • Yuluo Wu
    • Haiyang Zhao
  • View Affiliations

  • Published online on: September 27, 2021     https://doi.org/10.3892/ol.2021.13071
  • Article Number: 810
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Drug resistance is a key factor affecting the treatment of gastric cancer. The resistance of gastric cancer cells to anticancer drugs, such as cisplatin (DDP), remains a major challenge to patient recovery. The present study aimed to investigate the roles of C‑terminal‑binding protein 1 (CTBP1) in the DDP resistance of gastric cancer cells and to determine its regulatory effect on DNA repair protein RAD51 homolog 1 (RAD51). The DDP‑resistant human gastric cancer AGS and HGC cell lines, AGS/DDP and HGC‑27/DDP, respectively, were established and CTBP1 expression was detected by western blotting. In addition, Cell Counting Kit‑8, colony formation and flow cytometry assays were performed to detect the proliferation and apoptosis of these two cell lines following CTBP1 knockdown. The expression levels of apoptosis‑related proteins were detected by western blotting. In addition, RAD51 was overexpressed in CTBP1 knockdown cells, and proliferation and apoptosis were subsequently determined using the aforementioned methods. The results demonstrated that CTBP1 expression was notably increased in DDP‑resistant gastric cancer cells. Furthermore, CTBP1 knockdown suppressed the proliferation and induced the apoptosis of AGS/DDP and HGC‑27/DDP cells. Notably, CTBP1 promoted RAD51 expression in DDP‑resistant gastric cancer cells. Overexpression of RAD51 in CTBP1 knockdown AGS/DDP and HGC‑27/DDP cells rescued the proliferation and alleviated the apoptosis of these cells. Taken together, the results of the present study suggested that CTBP1 may enhance the DDP resistance of gastric cancer cells by activating RAD51 expression, thus providing a potential novel therapy (CTBP1 knockdown) for the clinical treatment of patients with gastric cancer.
View Figures
View References

Related Articles

Journal Cover

November-2021
Volume 22 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y and Zhao H: CTBP1 strengthens the cisplatin resistance of gastric cancer cells by upregulating RAD51 expression. Oncol Lett 22: 810, 2021.
APA
Wu, Y., & Zhao, H. (2021). CTBP1 strengthens the cisplatin resistance of gastric cancer cells by upregulating RAD51 expression. Oncology Letters, 22, 810. https://doi.org/10.3892/ol.2021.13071
MLA
Wu, Y., Zhao, H."CTBP1 strengthens the cisplatin resistance of gastric cancer cells by upregulating RAD51 expression". Oncology Letters 22.5 (2021): 810.
Chicago
Wu, Y., Zhao, H."CTBP1 strengthens the cisplatin resistance of gastric cancer cells by upregulating RAD51 expression". Oncology Letters 22, no. 5 (2021): 810. https://doi.org/10.3892/ol.2021.13071