G<sub>2</sub>/M cell cycle arrest and apoptosis induced by COH‑203 in human promyelocytic leukemia HL‑60 cells

Lin,Jihong; Zhang,Lei; Wang,Zhiwei; Guan,Qi; Bao,Kai; Wu,Lan

doi:10.3892/ol.2021.13076

G₂/M cell cycle arrest and apoptosis induced by COH‑203 in human promyelocytic leukemia HL‑60 cells

Authors:
- Jihong Lin
- Lei Zhang
- Zhiwei Wang
- Qi Guan
- Kai Bao
- Lan Wu
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Affiliations: Department of Geratology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China, Key Laboratory of Structure‑Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China
Published online on: September 28, 2021 https://doi.org/10.3892/ol.2021.13076
Article Number: 815
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The combretastatin A‑4/oltipraz hybrid (COH), 5‑(3‑amino‑4‑methoxyphenyl)‑4‑(3,4,5‑trimethoxyphenyl)‑3H‑1,2‑dithiole‑3‑one (COH‑203) is one of the COH compounds synthesized by our previous study, which has been reported to affect a number of cancer cell lines, such as SGC‑7901, KB, HT‑1080, HepG2, SMMC‑7721 and BEL‑7402. The sensitivity of human acute leukemia cell lines to COH‑203, and the mechanism underlying its anti‑proliferative effects remain unknown, which was investigated in the present study. In the present study, it was demonstrated that COH‑203 had notable time‑ and dose‑dependent antiproliferative effects on the human acute promyelocytic leukemia HL‑60 cell line. Furthermore, COH‑203 treatment resulted in cell cycle arrest at G₂/M phase in a dose‑dependent manner, and subsequently induced apoptosis. Western blot analysis revealed that upregulation of cyclin B was associated with G₂/M arrest. In addition, treatment with COH‑203 resulted in downregulated expression of Bcl‑2. This result revealed that COH‑203‑induced apoptosis in HL‑60 cells may occur via the mitochondrial pathway in a caspase‑dependent manner.

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