Clinical significance and effect of lncRNA BBOX1‑AS1 on the proliferation and migration of lung squamous cell carcinoma

Zhang,Yu; Wang,Xiao; Cheng,Xian-Kui; Zong,Yuan-Yuan; He,Rong-Quan; Chen,Gang; Qin,Ye-Jun

doi:10.3892/ol.2021.13135

Clinical significance and effect of lncRNA BBOX1‑AS1 on the proliferation and migration of lung squamous cell carcinoma

Authors:
- Yu Zhang
- Xiao Wang
- Xian-Kui Cheng
- Yuan-Yuan Zong
- Rong-Quan He
- Gang Chen
- Ye-Jun Qin
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Affiliations: Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250000, P.R. China, Department of Orthopedics, Shandong Second Provincial General Hospital, Shandong Provincial ENT Hospital, Jinan, Shandong 250000, P.R. China, Department of Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China
Published online on: November 12, 2021 https://doi.org/10.3892/ol.2021.13135
Article Number: 17
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) have a role in the occurrence and development of lung squamous cell carcinoma (LUSC). lncRNA γ‑butyrobetaine hydroxylase 1 (BBOX1)‑antisense 1 (AS1) may contribute to disease development. However, there are no studies on the role of BBOX1‑AS1 in LUSC to date. In the present study, an in‑house gene microarray analysis was performed to detect the differentially expressed lncRNAs and mRNAs between three pairs of LUSC and normal lung tissues. Only one lncRNA, BBOX1‑AS1, was differentially expressed in the in‑house microarray and The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and ArrayExpress databases. Reverse transcription‑quantitative PCR (RT‑qPCR) was then performed and the original RNA‑sequencing data from the TCGA, GEO and ArrayExpress datasets were used to determine the expression and clinical value of BBOX1‑AS1 in LUSC. In addition, a Cell Counting Kit‑8 assay, cell cycle analysis and scratch assay were performed to explore whether BBOX1‑AS1 expression affected the proliferation and migration of LUSC cells in vitro. The results of the RT‑qPCR analysis and data obtained from the TCGA database, GEO datasets, in‑house gene microarray and standard mean deviation analysis all supported the upregulated expression level of BBOX1‑AS1 in LUSC. Furthermore, silencing of BBOX1‑AS1 inhibited the proliferation and migration of LUSC cells according to in vitro assays. In addition, the cells were arrested in S‑phase after knockdown of BBOX1‑AS1. In conclusion, the expression level of BBOX1‑AS1 was upregulated in LUSC tissues. BBOX1‑AS1 may exert an oncogenic effect on LUSC by regulating various biological functions. However, additional functional experiments should be performed to verify the exact mechanism.

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