Open Access

Clinical significance and effect of lncRNA BBOX1‑AS1 on the proliferation and migration of lung squamous cell carcinoma

  • Authors:
    • Yu Zhang
    • Xiao Wang
    • Xian-Kui Cheng
    • Yuan-Yuan Zong
    • Rong-Quan He
    • Gang Chen
    • Ye-Jun Qin
  • View Affiliations

  • Published online on: November 12, 2021     https://doi.org/10.3892/ol.2021.13135
  • Article Number: 17
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) have a role in the occurrence and development of lung squamous cell carcinoma (LUSC). lncRNA γ‑butyrobetaine hydroxylase 1 (BBOX1)‑antisense 1 (AS1) may contribute to disease development. However, there are no studies on the role of BBOX1‑AS1 in LUSC to date. In the present study, an in‑house gene microarray analysis was performed to detect the differentially expressed lncRNAs and mRNAs between three pairs of LUSC and normal lung tissues. Only one lncRNA, BBOX1‑AS1, was differentially expressed in the in‑house microarray and The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and ArrayExpress databases. Reverse transcription‑quantitative PCR (RT‑qPCR) was then performed and the original RNA‑sequencing data from the TCGA, GEO and ArrayExpress datasets were used to determine the expression and clinical value of BBOX1‑AS1 in LUSC. In addition, a Cell Counting Kit‑8 assay, cell cycle analysis and scratch assay were performed to explore whether BBOX1‑AS1 expression affected the proliferation and migration of LUSC cells in vitro. The results of the RT‑qPCR analysis and data obtained from the TCGA database, GEO datasets, in‑house gene microarray and standard mean deviation analysis all supported the upregulated expression level of BBOX1‑AS1 in LUSC. Furthermore, silencing of BBOX1‑AS1 inhibited the proliferation and migration of LUSC cells according to in vitro assays. In addition, the cells were arrested in S‑phase after knockdown of BBOX1‑AS1. In conclusion, the expression level of BBOX1‑AS1 was upregulated in LUSC tissues. BBOX1‑AS1 may exert an oncogenic effect on LUSC by regulating various biological functions. However, additional functional experiments should be performed to verify the exact mechanism.
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January-2022
Volume 23 Issue 1

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Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang Y, Wang X, Cheng X, Zong Y, He R, Chen G and Qin Y: Clinical significance and effect of lncRNA BBOX1‑AS1 on the proliferation and migration of lung squamous cell carcinoma. Oncol Lett 23: 17, 2022.
APA
Zhang, Y., Wang, X., Cheng, X., Zong, Y., He, R., Chen, G., & Qin, Y. (2022). Clinical significance and effect of lncRNA BBOX1‑AS1 on the proliferation and migration of lung squamous cell carcinoma. Oncology Letters, 23, 17. https://doi.org/10.3892/ol.2021.13135
MLA
Zhang, Y., Wang, X., Cheng, X., Zong, Y., He, R., Chen, G., Qin, Y."Clinical significance and effect of lncRNA BBOX1‑AS1 on the proliferation and migration of lung squamous cell carcinoma". Oncology Letters 23.1 (2022): 17.
Chicago
Zhang, Y., Wang, X., Cheng, X., Zong, Y., He, R., Chen, G., Qin, Y."Clinical significance and effect of lncRNA BBOX1‑AS1 on the proliferation and migration of lung squamous cell carcinoma". Oncology Letters 23, no. 1 (2022): 17. https://doi.org/10.3892/ol.2021.13135